A requirement for NF-kappaB induction in the production of replication-competent HHV-8 virions

Oncogene
Marco SgarbantiJohn Hiscott

Abstract

The gammaherpesvirus human herpesvirus 8 (HHV-8) infects endothelial and B-lymphoid cells and is responsible for the development of Kaposi's sarcoma and primary effusion lymphoma (PEL). In the present study, we demonstrate that the activation of the NF-kappaB pathway during HHV-8 lytic replication is required for the generation of replication-competent virions capable of initiating a de novo infection of endothelial cells. In the HHV-8-positive PEL cell line BCBL-1, tetradecanoyl phorbol acetate (TPA) induction of the lytic cycle activates the NF-kappaB pathway, and this activation requires the induction of the IKKbeta component of the classical IkappaB kinase (IKK) complex. To further investigate the role of NF-kappaB activation in HHV-8 lytic replication, the NF-kappaB super-repressor IkappaBalpha-2NDelta4 was introduced into BCBL-1 cells by retroviral transduction. Expression of IkappaBalpha-2NDelta4 completely abolished NF-kappaB activity, as demonstrated by the loss of NF-kappaB DNA-binding activity and the absence of expression of the endogenous, NF-kappaB-regulated IkappaBalpha gene. NF-kappaB blockade dramatically impaired the ability of HHV-8 to produce infectious particles capable of initiating an effective de novo in...Continue Reading

References

Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C J EdgellJ B Graham
May 3, 1996·The Journal of Biological Chemistry·P BeauparlantJ Hiscott
Feb 11, 1999·Journal of Virology·R SunG Miller
Jun 29, 1999·Advances in Virus Research·R SaridP S Moore
Jun 3, 2000·Annual Review of Immunology·M Karin, Y Ben-Neriah
Feb 13, 2001·The Journal of Clinical Investigation·J HiscottP Génin
Nov 16, 2002·Science·Malini ChatterjeePatrick S Moore

❮ Previous
Next ❯

Citations

Aug 1, 2012·The Journal of Biological Chemistry·Aarthi NarayananFatah Kashanchi
Jun 1, 2011·Advances in Virology·Fengchun YeShou-Jiang Gao
Jun 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Leon Bernal-MizrachiLee Ratner
May 11, 2010·Trends in Microbiology·Deilson Elgui de OliveiraEthel Cesarman
Aug 1, 2006·Thrombosis Research·Sanjeev K Sahni
Nov 15, 2011·Biochemical and Biophysical Research Communications·Chiaki SajiMasahiro Fujimuro
May 6, 2014·Frontiers in Microbiology·Negin N BlattmanLawrence Corey
Jan 8, 2014·Molecular and Cellular Biology·Marco SgarbantiAngela Battistini
Jun 28, 2019·Journal of Virology·Chiara AcchioniMarco Sgarbanti

❮ Previous
Next ❯

Related Concepts

Related Feeds

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.