A reservoir of 'historical' antibiotic resistance genes in remote pristine Antarctic soils

Microbiome
Marc W Van GoethemThulani P Makhalanyane

Abstract

Soil bacteria naturally produce antibiotics as a competitive mechanism, with a concomitant evolution, and exchange by horizontal gene transfer, of a range of antibiotic resistance mechanisms. Surveys of bacterial resistance elements in edaphic systems have originated primarily from human-impacted environments, with relatively little information from remote and pristine environments, where the resistome may comprise the ancestral gene diversity. We used shotgun metagenomics to assess antibiotic resistance gene (ARG) distribution in 17 pristine and remote Antarctic surface soils within the undisturbed Mackay Glacier region. We also interrogated the phylogenetic placement of ARGs compared to environmental ARG sequences and tested for the presence of horizontal gene transfer elements flanking ARGs. In total, 177 naturally occurring ARGs were identified, most of which encoded single or multi-drug efflux pumps. Resistance mechanisms for the inactivation of aminoglycosides, chloramphenicol and β-lactam antibiotics were also common. Gram-negative bacteria harboured most ARGs (71%), with fewer genes from Gram-positive Actinobacteria and Bacilli (Firmicutes) (9%), reflecting the taxonomic composition of the soils. Strikingly, the abundan...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Nov 5, 1999·Applied and Environmental Microbiology·D N MillerW C Ghiorse
Jun 26, 2001·Research in Microbiology·C F Higgins
Mar 28, 2002·Nucleic Acids Research·A J EnrightC A Ouzounis
Jul 24, 2002·Nucleic Acids Research·Kazutaka KatohTakashi Miyata
Jan 14, 2004·Drugs·Xian-Zhi Li, Hiroshi Nikaido
May 26, 2004·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Barry G Hall, Miriam Barlow
Jun 11, 2004·Annual Review of Biochemistry·Amy L Davidson, Jue Chen
Jan 21, 2006·Science·Vanessa M D'CostaGerard D Wright
May 17, 2006·Ecotoxicology·Maria-Judith De SouzaD Chandramohan
Apr 5, 2008·Science·Gautam DantasGeorge M Church
Oct 4, 2008·Nucleic Acids Research·Bo Liu, Mihai Pop
Oct 10, 2008·The ISME Journal·Heather K AllenJo Handelsman
Nov 28, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Helen W BoucherJohn Bartlett
Jun 10, 2009·Bioinformatics·Salvador Capella-GutiérrezToni Gabaldón
Jun 30, 2009·Environmental Pollution·Jose Luis Martinez
Dec 1, 2009·Nature Reviews. Microbiology·Michael E HibbingS Brook Peterson
Mar 2, 2010·Nature Reviews. Microbiology·Heather K AllenJo Handelsman
Mar 9, 2010·Nature Reviews. Microbiology·Dan I Andersson, Diarmaid Hughes
Mar 10, 2010·BMC Bioinformatics·Doug HyattLoren J Hauser
Jul 16, 2010·DNA and Cell Biology·Patrick D SchlossJo Handelsman
Feb 1, 2011·Bioinformatics·Robert Schmieder, Robert Edwards
Jul 27, 2011·Chemical Reviews·Diego RomeroRoberto Kolter
Sep 2, 2011·Nature·Vanessa M D'CostaGerard D Wright
Sep 7, 2011·Trends in Microbiology·Don A CowanWarwick F Vincent
Sep 10, 2011·Bioinformatics·Tanja Magoč, Steven L Salzberg
Apr 18, 2012·PloS One·Kirandeep BhullarGerard D Wright
Sep 1, 2012·Science·Kevin J ForsbergGautam Dantas
Dec 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Noah FiererJ Gregory Caporaso
Jun 13, 2013·Environmental Microbiology Reports·Takahiro SegawaKazunari Ushida
Oct 15, 2013·Microbial Ecology·Eric M BottosStephen C Cary
May 13, 2014·Current Biology : CB·Joseph NesmePascal Simonet
May 23, 2014·Nature·Kevin J ForsbergGautam Dantas
Nov 18, 2014·Nature Methods·Benjamin BuchfinkDaniel H Huson
Nov 28, 2014·Nucleic Acids Research·Michael Y GalperinEugene V Koonin
Apr 25, 2015·Nature Communications·Jonathan S LefcheckJ Emmett Duffy
Nov 29, 2015·Bioinformatics·Alla MikheenkoAlexey Gurevich
Jan 8, 2016·FEMS Microbiology Ecology·David Fitzpatrick, Fiona Walsh
Feb 7, 2016·FEMS Microbiology Ecology·Ke-Qing XiaoYong-Guan Zhu

❮ Previous
Next ❯

Citations

Aug 1, 2018·Antimicrobial Agents and Chemotherapy·Karen Bush
Jun 6, 2020·International Journal of Environmental Research and Public Health·Laura C ScottTiong Gim Aw
Sep 12, 2020·Journal of Applied Microbiology·S JebriF Hmaied
May 20, 2020·Science China. Life Sciences·Jian CaoGeorge Fu Gao
Jul 14, 2020·Frontiers in Microbiology·María Florencia PerezJulián Rafael Dib
Mar 5, 2019·Frontiers in Microbiology·Sam LambrechtsGuillaume Tahon
Jan 6, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hassan WaseemMuhammad Ishtiaq Ali
Nov 14, 2019·Antonie van Leeuwenhoek·Eduarda AlmeidaOlga M Lage
Oct 15, 2019·Frontiers in Cellular and Infection Microbiology·Andrés Opazo-CapurroGerardo González-Rocha
Aug 16, 2019·Clinical Microbiology Reviews·Joaquim Ruiz
Sep 26, 2019·Frontiers in Public Health·Rene S HendriksenPatrick F McDermott
Dec 6, 2019·Frontiers in Microbiology·Aabir BanerjiScott Keely
Mar 25, 2019·Frontiers in Microbiology·Mariusz CycońZofia Piotrowska-Seget
Jan 7, 2021·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Pervaiz AliAamer Ali Shah
Feb 1, 2019·Environment International·Clare M McCannDavid W Graham
Feb 17, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Cláudio E T ParenteOlaf Malm
Feb 28, 2021·Journal of Environmental Sciences (China)·Hongjie WangChangping Yu
Nov 26, 2018·International Journal of Antimicrobial Agents·Guillaume André DurandGrégory Dubourg
Apr 27, 2021·Critical Reviews in Microbiology·Scott MitchellNicholas V Coleman
Apr 19, 2021·The Science of the Total Environment·Anh Q NguyenLong D Nghiem
Mar 3, 2020·The Science of the Total Environment·Yashini NaidooDon A Cowan

❮ Previous
Next ❯

Methods Mentioned

BETA
acetylation
MDS

Software Mentioned

CARD
MetaQUAST
vegan
Prodigal
trimAl
GeoChip
noradab
RPS
R
DIAMOND

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.