PMID: 9421961Jan 9, 1998Paper

A resonance Raman study of the C=C stretch modes in bovine and octopus visual pigments with isotopically labeled retinal chromophores

Photochemistry and Photobiology
L HuangR H Callender

Abstract

Previous resonance Raman spectroscopic studies of bovine and octopus rhodopsin and bathorhodopsin in the C-C stretch fingerprint region have shown drastically different spectral patterns, which suggest different chromophore-protein interactions. We have extended our resonance Raman studies of bovine and octopus pigments to the C=C stretch region in order to reveal a more detailed picture about the difference in retinal-protein interactions between these two pigments. The C=C stretch motions of the protonated retinal Schiff base are strongly coupled to form highly delocalized ethylenic modes located in the 1500 to 1650 cm-1 spectral region. In order to decouple these vibrations, a series of 11,12-D2-labeled retinals, with additional 13C labeling at C8, C10, C11 and C14, respectively, are used to determine the difference of specific C=C stretch modes between bovine and octopus pigments. Our results show that the C9=C10 and C13=C14 stretch mode are about 20 cm-1 lower in the Raman spectrum of octopus bathorhodopsin than in bovine bathorhodopsin, while the other C=C stretch modes in these two bathorhodopsins are similar. In contrast, only the C9=C10 stretch mode in octopus rhodopsin is about 10 cm-1 lower than in bovine rhodopsin, ...Continue Reading

References

Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D C Blazej, W L Peticolas
Jan 1, 1977·Annual Review of Biophysics and Bioengineering·A Warshel
Dec 1, 1991·Photochemistry and Photobiology·H DengR H Callender
Nov 17, 1987·Biochemistry·H Deng, R H Callender

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Citations

May 25, 2005·Journal of Biomedical Optics·Aiguo ShenJiming Hu
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·Thomas P SakmarElias S Awad
Jul 13, 2007·Skeletal Radiology·P J MullaneyD C Salonen
Oct 3, 2001·Physiological Reviews·S T MenonT P Sakmar
Jul 17, 1999·Analytical Chemistry·C K LariveS Bogdanowich-Knipp

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