A resource for the conditional ablation of microRNAs in the mouse.

Cell Reports
Chong Yon ParkMichael T McManus

Abstract

The importance of miRNAs during development and disease processes is well established. However, most studies have been done in cells or with patient tissues, and therefore the physiological roles of miRNAs are not well understood. To unravel in vivo functions of miRNAs, we have generated conditional, reporter-tagged knockout-first mice for numerous evolutionarily conserved miRNAs. Here, we report the generation of 162 miRNA targeting vectors, 64 targeted ES cell lines, and 46 germline-transmitted miRNA knockout mice. In vivo lacZ reporter analysis in 18 lines revealed highly tissue-specific expression patterns and their miRNA expression profiling matched closely with published expression data. Most miRNA knockout mice tested were viable, supporting a mechanism by which miRNAs act redundantly with other miRNAs or other pathways. These data and collection of resources will be of value for the in vivo dissection of miRNA functions in mouse models.

References

Mar 30, 2004·Genesis : the Journal of Genetics and Development·Giuseppe TestaA Francis Stewart
Sep 2, 2004·Nature Genetics·Christopher P AustinBrian Zambrowicz
Sep 2, 2004·Nature Genetics·Johan AuwerxWolfgang Wurst
May 28, 2005·Science·Erno WienholdsRonald H A Plasterk
Nov 1, 2005·Nature·Jan KrützfeldtMarkus Stoffel
Dec 22, 2005·Nature Methods·Wigard P KloostermanRonald H A Plasterk
Oct 3, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Diana K DarnellParker B Antin
Jan 16, 2007·Cell·Francis S CollinsWolfgang Wurst
Oct 31, 2007·Briefings in Functional Genomics & Proteomics·Roland H FriedelWolfgang Wurst
Dec 7, 2007·Cancer Biology & Therapy·Antonis GiannakakisLin Zhang
Feb 20, 2008·PLoS Genetics·Ivan OsokineMichael T McManus
Mar 5, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Carme CampsJiannis Ragoussis
Jan 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Krystian JazdzewskiAlbert de la Chapelle
Jan 26, 2010·Current Biology : CB·Ezequiel Alvarez-Saavedra, H Robert Horvitz
May 18, 2010·Molecular Cell·Haruhiko Siomi, Mikiko C Siomi
Sep 2, 2010·Human Molecular Genetics·Chong Y ParkMichael T McManus
Oct 28, 2010·The Journal of Clinical Investigation·David M PatrickEric N Olson
Oct 29, 2010·Journal of Biomedicine & Biotechnology·Mikhail NefedovPieter J de Jong
Aug 9, 2011·Nature Biotechnology·Haydn M ProsserAllan Bradley

❮ Previous
Next ❯

Citations

Mar 19, 2013·Trends in Immunology·Mario Leonardo SquadritoMikael J Pittet
Jun 12, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Rasmus O BakJacob Giehm Mikkelsen
Jul 23, 2013·Nature Immunology·Silvia Monticelli, Gioacchino Natoli
Jul 3, 2013·Nature Reviews. Genetics·Kailiang Sun, Eric C Lai
Nov 12, 2013·Nature Structural & Molecular Biology·Young-Kook KimV Narry Kim
Nov 24, 2012·Nucleic Acids Research·Carol J BultUNKNOWN Mouse Genome Database Group
May 26, 2012·PloS One·Lukas T JekerJeffrey A Bluestone
Jan 12, 2013·PLoS Pathogens·Rodney P Kincaid, Christopher S Sullivan
Mar 15, 2013·Proceedings of the National Academy of Sciences of the United States of America·Chunliang LiCharles J Sherr
Jul 16, 2014·Nature Reviews. Genetics·Jean Hausser, Mihaela Zavolan
Sep 6, 2014·Cell Death and Differentiation·A Wilczynska, M Bushell
Nov 20, 2014·Science Signaling·Viraj R SanghviHans-Guido Wendel
Aug 3, 2013·Nature Reviews. Immunology·Dirk Baumjohann, K Mark Ansel
Sep 18, 2012·Cellular and Molecular Life Sciences : CMLS·Matthew S Ning, Thomas Andl
Jul 24, 2013·Annual Review of Cell and Developmental Biology·Tobias S GreveRobert Blelloch
Jan 3, 2015·Advanced Drug Delivery Reviews·Kelsey R BeaversCraig L Duvall
Aug 28, 2015·International Journal of Obesity : Journal of the International Association for the Study of Obesity·J A Deiuliis
Aug 9, 2015·Brain Research·Mary P Heyer, Paul J Kenny
Mar 8, 2016·Wiley Interdisciplinary Reviews. Developmental Biology·Bruno R SteinkrausTudor A Fulga
Apr 5, 2013·Immunological Reviews·Lukas T Jeker, Jeffrey A Bluestone
Apr 5, 2013·Immunological Reviews·Sara MontagnerSilvia Monticelli
Apr 5, 2013·Immunological Reviews·Aimee M BeaulieuLewis L Lanier
Jun 12, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Gary Gibson, Hiroshi Asahara
Oct 3, 2015·American Journal of Obstetrics and Gynecology·Jean-François MouilletYoel Sadovsky
Jul 14, 2014·Biological Psychiatry·James J CrowleyPatrick F Sullivan
May 30, 2015·Current Opinion in Pharmacology·Lukas T Jeker, Romina Marone
Jun 18, 2015·Nature Communications·Tudor A FulgaDavid Van Vactor
Aug 14, 2012·Neuron·Elizabeth McNeill, David Van Vactor
Oct 4, 2012·Current Opinion in Immunology·Joerg Ermann, Laurie H Glimcher
Dec 9, 2014·Trends in Cell Biology·Joana A Vidigal, Andrea Ventura
May 31, 2014·Cell and Tissue Research·Dietrich Trümbach, Nilima Prakash
May 16, 2014·Cell and Tissue Research·Frederick RehfeldF Gregory Wulczyn

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Sequencing

Several sequencing approaches are employed to understand and examine tumor development and progression. These include whole genome as well as RNA sequencing. Here is the latest research on cancer sequencing.

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.