A response-regulator-like activator of antibiotic synthesis from Streptomyces coelicolor A3(2) with an amino-terminal domain that lacks a phosphorylation pocket.

Microbiology
E P GuthrieK F Chater

Abstract

In Streptomyces coelicolor A3(2), bldA mutants that lack the tRNA for the rare leucine codon UUA fail to make the red undecylprodigiosin antibiotic complex. To find out why, red-pigmented while bald (Pwb) derivatives of a bldA mutant were isolated. Using a cloning strategy that allowed for (and demonstrated) dominance of the mutations, they were localized to the red gene cluster. By using insert-mediated integration of a phi C31 phage-based vector, one of the Pwb mutations was more precisely located between red structural genes to a segment of approximately 1 kb about 4 kb from the known pathway-specific regulatory gene redD. The segment contained most of an ORF (redZ) encoding a protein (RedZ) with end-to-end similarity to response regulators of diverse function from a variety of bacteria. Remarkably, in RedZ hydrophobic residues replace nearly all of the charged residues that usually make up the phosphorylation pocket present in typical response regulators, including the aspartic acid residue that is normally phosphorylated by a cognate sensory protein kinase. A single TTA codon in redZ provided a potential explanation for the bldA-dependence of undecylprodigiosin synthesis. This codon was unchanged in three Pwb mutants, but ...Continue Reading

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Citations

Jan 31, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sheenu Mittal, Lee Kroos
Sep 8, 2015·Journal of Food Science and Technology·N Darshan, H K Manonmani
May 9, 2009·Proceedings of the National Academy of Sciences of the United States of America·Linqi WangHuarong Tan
Nov 30, 2005·Molecular Microbiology·Jianqiang HuangStanley N Cohen
May 4, 2018·Natural Product Reports·Helga U van der HeulGilles P van Wezel
Mar 9, 2013·Microbiology and Molecular Biology Reviews : MMBR·Gang LiuHuarong Tan
Nov 17, 2006·Nature Reviews. Microbiology·Neil R WilliamsonGeorge P C Salmond
Jun 16, 2010·Molecular Microbiology·Katherina ZakikhanyUte Römling
Jan 20, 2006·Molecular Microbiology·Joanne M WilleyJustin R Nodwell
Sep 25, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xuri WuYijun Chen
Sep 23, 2016·Molecular Microbiology·Stuti K Desai, Linda J Kenney
Mar 20, 2018·Applied Microbiology and Biotechnology·Bingbing HouHuizhan Zhang
Aug 9, 2017·Science China. Life Sciences·Panpan LiuYinhua Lu
Sep 8, 2010·Journal of Bacteriology·Fatma KarrayJean-Luc Pernodet

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