A Retrospective Study about the Impact of Switching from Nested PCR to Multiplex Real-Time PCR on the Distribution of the Human Papillomavirus (HPV) Genotypes

Medicina
Raffaele Del PreteLuigi Santacroce

Abstract

Human papillomavirus (HPV) is the most prevalent etiological agent of viral sexually-transmitted infection. This study retrospectively evaluated the impact of a switch to a real-time PCR assay in the HPV prevalence and genotypes distribution by a quasi-experimental before-and-after approach. In total, 1742 samples collected from 1433 patients were analyzed at the UOC Microbiology and Virology of Policlinico of Bari, Italy. HPV DNA detection was performed using initially nested PCR and subsequently multiplex real-time PCR assay. Statistically significant difference in HPV overall prevalence after the introduction of the real-time assay was not detected (48.97% vs. 50.62%). According to different extraction-DNA amplification methods, differences were observed in the prevalence rates of HPV-45, 68, 40, 42, and 43. The lowest prevalence for HPV-45 was observed in the Magna Pure-Real Time PCR group, while HPV-68, 40, 42, and 43 were less observed in the Qiagen-Real Time PCR group. After, a multivariate logistic regression, an increase in the prevalence of HPV-42 (aOR: 4.08, 95% CI: 1.71-9.73) was associated with the multiplex real-time PCR assay. Although this study is a not a direct comparison between two diagnostic methods because...Continue Reading

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Citations

May 7, 2020·International Journal of Environmental Research and Public Health·Francesco InchingoloIoannis Alexandros Charitos
Oct 19, 2019·Endocrine, Metabolic & Immune Disorders Drug Targets·Luigi SantacroceIoannis Alexandros Charitos
Jan 26, 2020·Cancers·Skender TopiFrancesco Inchingolo
Apr 4, 2021·Viruses·Luigi SantacroceGianna Dipalma

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Methods Mentioned

BETA
PCR
electrophoresis
genotyping
Assay

Software Mentioned

MolecuTech
Multivariate Imputation by Chained Equations ( MICE )
Euroarray
R
AnyplexTM
Magna Pure - Real Time
Anyplex

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