A reverse catalytic triad Asp containing loop shaping a wide substrate binding pocket of a feruloyl esterase from Lactobacillus plantarum.

International Journal of Biological Macromolecules
Haowen ZhangFengjiao Xin

Abstract

Feruloyl esterase is an indispensable biocatalyst in food processing, pesticide and pharmaceutical industries, catalyzing the cleavage of the ester bond cross-linked between the polysaccharide side chain of hemicellulose and ferulic acid in plant cell walls. LP_0796 from Lactobacillus plantarum was identified as a feruloyl esterase that may have potential applications in the food industry, but the lack of the substrate recognition and catalytic mechanisms limits its application. Here, LP_0796 showed the highest activity towards methyl caffeate at pH 6.6 and 40 °C. The crystal structure of LP_0796 was determined at 2.5 Å resolution and featured a catalytic triad Asp195-containing loop facing the opposite direction, thus forming a wider substrate binding pocket. Molecular docking simulation and site-directed mutagenesis studies further demonstrated that in addition to the catalytic triad (Ser94, Asp195, His225), Arg125 and Val128 played essential roles in the function of the active site. Our data also showed that Asp mutation of Ala23 and Ile198 increased the catalytic efficiency to 4- and 5-fold, respectively. Collectively, this work provided a better understanding of the substrate recognition and catalytic mechanisms of LP_0796...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Nov 5, 1999·Journal of Molecular Biology·S Kumar, R Nussinov
Mar 21, 2002·Journal of Agricultural and Food Chemistry·Hiroe KikuzakiHisaji Taniguchi
Aug 31, 2002·Biophysical Journal·Sandeep Kumar, Ruth Nussinov
Oct 24, 2002·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsThomas C Terwilliger
Dec 9, 2003·Applied Microbiology and Biotechnology·V F CrepinI F Connerton
Apr 7, 2004·Applied and Environmental Microbiology·Xiaokun WangHiroo Sanada
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Jul 16, 2005·Proteins·James N Sarakatsannis, Yong Duan
May 16, 2006·Carbohydrate Research·Isabelle BenoitMichèle Asther
Oct 18, 2006·Applied Microbiology and Biotechnology·Hyun-Dong Shin, Rachel Ruizhen Chen
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
May 12, 2010·Nucleic Acids Research·Liisa Holm, Päivi Rosenström
Jan 18, 2011·Journal of Molecular Graphics & Modelling·Rajender KumarRakesh Yadav
Jan 1, 2000·Journal of Biomolecular Structure & Dynamics·S KumarR Nussinov
Oct 12, 2012·Molecular Biotechnology·Inmaculada Navarro-GonzálezGarcía-Carmona Francisco
Jun 25, 2013·Applied and Environmental Microbiology·María Esteban-TorresRosario Muñoz
Apr 23, 2014·Nucleic Acids Research·Xavier Robert, Patrice Gouet
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor
Nov 16, 2017·Current Opinion in Structural Biology·Anna PabisShina Cl Kamerlin
Jul 20, 2018·Journal of the Royal Society, Interface·Dušan PetrovićJose M Sanchez-Ruiz
Feb 2, 2019·Bioresource Technology·Dyoni M OliveiraWanderley D Dos Santos
Sep 29, 2019·The Journal of Biological Chemistry·Jesper HolckCasper Wilkens
Oct 3, 2019·Planta·Marcia M de O BuanafinaHoward W Fescemyer
Jan 1, 2021·International Journal of Biological Macromolecules·Jiyoung ParkKyung-Jin Kim

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