A Reverse Genetics System for the Israeli Acute Paralysis Virus and Chronic Bee Paralysis Virus

International Journal of Molecular Sciences
Sa YangChunsheng Hou

Abstract

Honey bee viruses are associated with honey bee colony decline. Israeli acute paralysis virus (IAPV) is considered to have a strong impact on honey bee survival. Phylogenetic analysis of the viral genomes from several regions of the world showed that various IAPV lineages had substantial differences in virulence. Chronic bee paralysis virus (CBPV), another important honey bee virus, can induce two significantly different symptoms. However, the infection characteristics and pathogenesis of IAPV and CBPV have not been completely elucidated. Here, we constructed infectious clones of IAPV and CBPV using a universal vector to provide a basis for studying their replication and pathogenesis. Infectious IAPV and CBPV were rescued from molecular clones of IAPV and CBPV genomes, respectively, that induced typical paralysis symptoms. The replication levels and expression proteins of IAPV and CBPV in progeny virus production were confirmed by qPCR and Western blot. Our results will allow further dissection of the role of each gene in the context of viral infection while helping to study viral pathogenesis and develop antiviral drugs using reverse genetics systems.

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Citations

Jun 18, 2020·Frontiers in Microbiology·Orlando YañezNor Chejanovsky
Aug 11, 2021·Environmental Science & Technology·Yanchun DengChunsheng Hou

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Datasets Mentioned

BETA
MG599488
MF175173
MF175174

Methods Mentioned

BETA
PCR
in vitro transcription
electrophoresis

Software Mentioned

GraphPad Prism

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