PMID: 11906730Mar 22, 2002Paper

A reverse-transcription competitive PCR assay based on chemiluminescence hybridization for detection and quantification of hepatitis C virus RNA

Journal of Virological Methods
Kung-Chia YoungChung-Min Jen

Abstract

A reverse-transcription competitive PCR (RT-cPCR) combined with chemiluminescence hybridization was designed for the detection and quantitative determination of serum hepatitis C virus (HCV) RNA. The concentration of HCV RNA was calculated based on an external standard curve that was generated by coamplification of internal competitor and target sequences in serial dilutions. The detection limit of the chemiluminescence RT-cPCR was 100 copies/ml (94 IU/ml). Meanwhile, the linear range for quantitation extended from 850 copies/ml (795 IU/ml) to 4.95x10(7) copies/ml. The performance of the current assay for measuring circulating HCV levels from 26 anti-HCV-antibody positive patients was compared with that of branched-chain DNA (bDNA) and nested RT-PCR assays. Eighteen patients had HCV RNA levels that exceeded the quantitation limit by the chemiluminescence RT-cPCR, but only 11 patients were quantitation-positive by the bDNA. A significant correlation of the quantitation values was found between the chemiluminescence RT-cPCR and the bDNA (R2=0.8391). Among the eight patients with HCV RNA titers below the quantitation limit, four remained positive by the chemiluminescence cRT-PCR, demonstrating the results in agreement with those u...Continue Reading

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Citations

Nov 5, 2003·Analytical Biochemistry·Zhen Huang, Mohammed Alsaidi
Sep 27, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kung-Chia YoungMichael M C Lai
Dec 8, 2006·Sexually Transmitted Infections·R Gilson, M G Brook
Jan 20, 2016·Analytical and Bioanalytical Chemistry·Il Young JungHyukjin Lee
Sep 8, 2004·Journal of Virological Methods·Ye-Fu WangHui-Hui Liu
Apr 4, 2003·Analytical Biochemistry·Zhen Huang, Jack W Szostak
Jun 4, 2011·FEMS Immunology and Medical Microbiology·Qin-qin WangYong-sheng Liu
Mar 4, 2006·Journal of Virological Methods·Valérie Balme-SinibaldiEmmanuel Jacquot
Aug 10, 2004·Chembiochem : a European Journal of Chemical Biology·Mohammed AlsaidiZhen Huang
Jun 1, 2007·Luminescence : the Journal of Biological and Chemical Luminescence·Jiu-Cun WangZewei Luo

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