A reversed-phase/hydrophilic interaction mixed-mode C18-Diol stationary phase for multiple applications

Analytica Chimica Acta
Qing WangZhi-guo Shi

Abstract

A mixed-mode chromatographic packing material, C18 and diol groups modified silica (C18-Diol), was prepared with controllable hydrophobicity and hydrophilicity. It demonstrated excellent aqueous compatibility and stability in aqueous mobile phase; compared to the traditional C18 column, improved peak shape of basic analytes was also obtained. Additionally, it exhibited both reversed-phase liquid chromatographic (RPLC) and hydrophilic interaction chromatographic (HILIC) performance; the analyte separation scope was thus enlarged, demonstrated by simultaneous separation of twenty acids, bases and neutrals. More interestingly, a novel on-line two-dimensional liquid chromatography on the single column (2D-LC-1C) was established by modifying the high performance liquid chromatographic instrument only with the addition of an extra six-port two-position valve. The early co-eluted components of the extract of Lonicera japonica on the 1st-dimension (RPLC) were collected for the online re-injection to the 2nd-dimension (HILIC) by conveniently varying the mobile phase components. Six more peaks were obtained. The established system was simple, easy operation and low cost, which had advantages in analyzing complicated samples.

References

Sep 25, 2007·Journal of Chromatography. a·Dwight R StollSarah C Rutan
Aug 1, 2008·Journal of Separation Science·Colin T Mant, Robert S Hodges
Jul 22, 2009·Journal of Chromatography. a·Peng LiuXindu Geng
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Mar 16, 2013·Journal of Separation Science·Qiaoli ZhouLi Jia

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Citations

May 29, 2016·Journal of Pharmaceutical and Biomedical Analysis·Kelly Zhang, Xiaodong Liu
Sep 21, 2016·Journal of Pharmaceutical and Biomedical Analysis·Kelly Zhang, Xiaodong Liu
Sep 17, 2017·Oncotarget·Zhi-Hua OuyangGuo-Hua Lv
Mar 15, 2018·Oncotarget·Jin-Cheng HuangHai Lu
Jan 16, 2021·Biomedical Chromatography : BMC·Elham Sadat Hosseini, Kourosh Tabar Heydar
Nov 10, 2020·Analytica Chimica Acta·Kenichi Nagase, Hideko Kanazawa

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