A reversibly immortalized human hepatocyte cell line as a source of hepatocyte-based biological support

Addiction Biology
Naoya KobayashiP Leboulch

Abstract

The application of hepatocyte transplantation (HTX) is increasingly envisioned for temporary metabolic support during acute liver failure and provision of specific liver functions in inherited liver-based metabolic diseases. Compared with whole liver transplantation, HTX is a technically simple procedure and hepatocytes can be cryopreserved for future use. A major limitation of this form of therapy in humans is the worldwide shortage of human livers for isolating an adequate number of transplantable human hepatocyes when needed. Furthermore, the numbers of donor livers available for hepatocyte isolation is limited by competition for their use in whole organ transplantation. Considering the cost of hepatocyte isolation and the need for immediate preparation of consistent and functional cells, it is unlikely that human hepatocytes can be obtained on such a scale to treat a large number of patients with falling liver functions. The utilization of xenogenic hepatocytes will result in additional concerns regarding transmission of infectious pathogens and immunological and physiological incompatibilities between animals and humans. An attractive alternative to primary human hepatocytes is the use of tightly regulated human hepatocyte...Continue Reading

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Citations

Aug 23, 2006·Expert Opinion on Drug Metabolism & Toxicology·Marc VermeirGeert Mannens
Oct 29, 2003·Cell Transplantation·Takemi KuniedaNaoya Kobayashi
May 13, 2003·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Umberto BaccaraniAnnibale Donini
Sep 28, 2004·Journal of Pediatric Gastroenterology and Nutrition·M Najimi, E Sokal
Aug 1, 2009·Langenbeck's Archives of Surgery·Sabrina EhnertAndreas Nussler
Nov 23, 2010·Frontiers of Medicine·Yi-Tao Ding, Xiao-Lei Shi
Apr 5, 2011·Journal of Materials Science. Materials in Medicine·Andreas K NusslerThomas S Weiss
Jan 12, 2019·World Journal of Stem Cells·Saifun NaharJiro Fujita
Nov 14, 2006·The International Journal of Biochemistry & Cell Biology·Demetra Mavri-DamelinClare Selden

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