A review on microfluidics manipulation of the extracellular chemical microenvironment and its emerging application to cell analysis.

Analytica Chimica Acta
Peng ChenBi-Feng Liu

Abstract

Spatiotemporal manipulation of extracellular chemical environments with simultaneous monitoring of cellular responses plays an essential role in exploring fundamental biological processes and expands our understanding of underlying mechanisms. Despite the rapid progress and promising successes in manipulation strategies, many challenges remain due to the small size of cells and the rapid diffusion of chemical molecules. Fortunately, emerging microfluidic technology has become a powerful approach for precisely controlling the extracellular chemical microenvironment, which benefits from its integration capacity, automation, and high-throughput capability, as well as its high resolution down to submicron. Here, we summarize recent advances in microfluidics manipulation of the extracellular chemical microenvironment, including the following aspects: i) Spatial manipulation of chemical microenvironments realized by convection flow-, diffusion-, and droplet-based microfluidics, and surface chemical modification; ii) Temporal manipulation of chemical microenvironments enabled by flow switching/shifting, moving/flowing cells across laminar flows, integrated microvalves/pumps, and droplet manipulation; iii) Spatiotemporal manipulation o...Continue Reading

Citations

Apr 16, 2021·Pflügers Archiv : European journal of physiology·Oisín KingCesare M Terracciano
Apr 13, 2021·Frontiers in Molecular Biosciences·Fabrizio MatteiGiovanna Schiavoni

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