A review on recent developments for biomolecule separation at analytical scale using microfluidic devices

Analytica Chimica Acta
Kishore K R Tetala, M A Vijayalakshmi

Abstract

Microfluidic devices with their inherent advantages like the ability to handle 10(-9) to 10(-18) L volume, multiplexing of microchannels, rapid analysis and on-chip detection are proving to be efficient systems in various fields of life sciences. This review highlights articles published since 2010 that reports the use of microfluidic devices to separate biomolecules (DNA, RNA and proteins) using chromatography principles (size, charge, hydrophobicity and affinity) along with microchip capillary electrophoresis, isotachophoresis etc. A detailed overview of stationary phase materials and the approaches to incorporate them within the microchannels of microchips is provided as well as a brief overview of chemical methods to immobilize ligand(s). Furthermore, we review research articles that deal with microfluidic devices as analytical tools for biomolecule (DNA, RNA and protein) separation.

References

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Citations

May 18, 2016·Biomicrofluidics·Radim KnobAdam T Woolley
Oct 11, 2016·Analytica Chimica Acta·Athina S KastaniaEvangelos Gogolides
Nov 3, 2016·Journal of Separation Science·Sille Štěpánová, Václav Kašička
Mar 18, 2017·Electrophoresis·Martina Viefhues, Ralf Eichhorn
May 31, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Austin W H Lee, Byron D Gates
Feb 23, 2018·Analytical and Bioanalytical Chemistry·Jimmy LyR Michael van Dam
Sep 6, 2017·Electrophoresis·Ales PodgornikShuichi Yamamoto
Nov 2, 2018·Analytical and Bioanalytical Chemistry·Ellen K ParkerAdam T Woolley
May 26, 2020·Critical Reviews in Analytical Chemistry·Marwa A A Ragab, Eman I El-Kimary
Dec 8, 2017·The Analyst·I Rodríguez-RuizJ A Gavira
Sep 15, 2019·Analytica Chimica Acta·María Vergara-BarberánJosé Manuel Herrero-Martínez
Dec 5, 2020·Scientific Reports·Vitaly V RyzhkovIlya A Rodionov
Jun 6, 2021·Trends in Microbiology·Nuno F AzevedoDarla M Goeres
Jun 18, 2021·Lab on a Chip·Mahmudur RahmanHolger Schmidt
May 15, 2019·Analytical Chemistry·Sebastian K PiendlDetlev Belder

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