A Rhipicephalus (Boophilus) microplus cathepsin with dual peptidase and antimicrobial activity

International Journal for Parasitology
Daiane P OldigesCarlos Termignoni

Abstract

The cattle tick, Rhipicephalus (Boophilus) microplus, is a haematophagous arthropod responsible for considerable losses in the livestock industry. Immunological control with vaccines is a promising alternative to replace chemical acaricides. Due to their importance in parasite physiology, cysteine endopeptidases are potential targets. In a previous study, native Vitellin Degrading Cysteine Endopeptidase (VTDCE) was successfully tested as a vaccine antigen for bovines against R. microplus. In this work, nucleotide and amino acid VTDCE sequences were obtained from cDNA databanks, based on data from Edman sequencing and mass spectrometry. Subsequently, cloning and expression, purification, immunological and biochemical characterisation of the recombinant protein were performed to determine the biological importance of VTDCE. By Western blot, polyclonal antibodies produced against recombinant VTDCE recognised native VTDCE. Interestingly, molecular analysis showed that the VTDCE sequence has similarity to antimicrobial peptides. Indeed, experimental results revealed that VTDCE has an antimicrobial activity which is independent of endopeptidase activity. We believe that this is the first known study to show that an arthropod enzyme h...Continue Reading

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Citations

Feb 5, 2016·Insect Biochemistry and Molecular Biology·Lucas TirloniItabajara da Silva Vaz
Apr 16, 2013·Ticks and Tick-borne Diseases·Karine R ZimmerCarlos Termignoni
Sep 8, 2020·The FEBS Journal·Deepani Darshika Fernando, Katja Fischer

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