A Rho-kinase inhibitor improves cardiac function after 24-hour heart preservation

The Journal of Thoracic and Cardiovascular Surgery
Mariko KobayashiRyuji Tominaga

Abstract

The Rho-kinase signaling pathway is associated with coronary vasculopathy and myocardial dysfunction after cardiac transplantation. This study evaluated whether using a Rho-kinase inhibitor during allograft storage could limit early endothelial dysfunction and improve myocardial performance after reperfusion. This experiment was performed with an isolated working rabbit heart model and a support rabbit. Donor hearts (control group, n = 8) were arrested with an extracellular type of cardioplegia, preserved with University of Wisconsin solution, and then immersed in University of Wisconsin solution for 24 hours (1 degrees C). The Rho-kinase inhibitor (Rho-kinase inhibitor group, n = 8) was administrated in the cardioplegic solution, the preservation University of Wisconsin solution, and the storage University of Wisconsin solution. Left ventricular performance was evaluated from the modified Frank-Starling curve in the working mode. Coronary blood flow and donor heart rate were measured in Langendorff mode. Effective evaluation of the Rho-kinase inhibitor was inferred from phosphorylated myosin light chain. The expression of endothelial nitric oxide synthase mRNA was analyzed to assess endothelial function. The Frank-Starling cur...Continue Reading

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Citations

Sep 21, 2011·Future Cardiology·Michelle SurmaJianjian Shi
Nov 30, 2013·The Annals of Thoracic Surgery·Go WatanabePham Minh Tuan
May 17, 2011·American Heart Journal·María Paz OcaranzaJorge E Jalil
Dec 30, 2014·Interactive Cardiovascular and Thoracic Surgery·Sarkis M MinasianTimur D Vlasov
Feb 10, 2010·Journal of Vascular Surgery·Hironori BabaRyuji Tominaga
Feb 4, 2010·Current Opinion in Organ Transplantation·Steven JacobsBart Meyns
Mar 29, 2019·Interactive Cardiovascular and Thoracic Surgery·Yuki NakamuraYoshiki Sawa
Aug 9, 2016·Transplantation Direct·Ryuta SeguchiShojiro Yamaguchi
Mar 14, 2019·Current Pharmaceutical Design·Fakhra AminBilqees Bano
Mar 31, 2010·Current Opinion in Organ Transplantation

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