A Rho signaling network links microtubules to PKD controlled carrier transport to focal adhesions

ELife
Stephan A EislerAngelika Hausser

Abstract

Protein kinase D (PKD) is a family of serine/threonine kinases that is required for the structural integrity and function of the Golgi complex. Despite its importance in the regulation of Golgi function, the molecular mechanisms regulating PKD activity are still incompletely understood. Using the genetically encoded PKD activity reporter G-PKDrep we now uncover a Rho signaling network comprising GEF-H1, the RhoGAP DLC3, and the Rho effector PLCε that regulate the activation of PKD at trans-Golgi membranes. We further show that this molecular network coordinates the formation of TGN-derived Rab6-positive transport carriers delivering cargo for localized exocytosis at focal adhesions.

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Citations

Oct 11, 2019·Journal of Cell Science·Shailaja Seetharaman, Sandrine Etienne-Manneville
Nov 21, 2019·International Journal of Cancer. Journal International Du Cancer·Wolfgang S LiebAngelika Hausser
May 31, 2019·The Journal of Cell Biology·Lou FourriereStéphanie Miserey-Lenkei
Jan 4, 2019·Nature Cell Biology·Paulina Moreno-LaysecaJohanna Ivaska
Nov 27, 2019·Cells·Monilola A OlayioyeAngelika Hausser
Nov 16, 2019·Journal of Cell Science·Florian WeeberTim Eiseler
Aug 6, 2019·Frontiers in Cell and Developmental Biology·Santosh Phuyal, Hesso Farhan
Jan 31, 2020·Journal of Cell Science·Lou FourriereGaelle Boncompain
Nov 5, 2019·Cancer Research·Dan GeorgessAndrew J Ewald
Oct 7, 2020·Trends in Biochemical Sciences·Martijn A NolteCoert Margadant
Nov 15, 2020·Nature Reviews. Molecular Cell Biology·Patrizia RomaniSirio Dupont
Sep 24, 2020·Progress in Lipid Research·Matilda Katan, Shamshad Cockcroft
Jul 18, 2020·Trends in Cell Biology·Shailaja Seetharaman, Sandrine Etienne-Manneville
Dec 7, 2021·Journal of Cell Science·Charlotte FordChristopher G Burd

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Methods Mentioned

BETA
GTPases
GTPase
transfection
Biosensor
FRET
dot blot
confocal microscopy
Profiler
FCS
PCR

Software Mentioned

Cell Profiler
GraphPad Prism
Image Studio Lite
Zen black
VisArt
CellProfiler

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