A Rhodnius prolixus Insulin Receptor and Its Conserved Intracellular Signaling Pathway and Regulation of Metabolism

Frontiers in Endocrinology
Marina S DefferrariAngela B Lange

Abstract

The insulin signaling pathway is a modulator of metabolism in insects and can regulate functions associated with growth and development, as well as lipid and carbohydrate balance. We have previously reported the presence of an insulin-like peptide and an insulin-like growth factor in Rhodnius prolixus, which are involved in the homeostasis of lipids and carbohydrates in post-feeding and non-feeding periods. In the present study, we have characterized the first insulin receptor (IR) to be discovered in R. prolixus, Rhopr-IR, and investigated its intracellular signaling cascade and its role in nutrient control. We identified a candidate protein sequence within R. prolixus putative peptidome and predicted its conserved features using bioinformatics. Tissue-specific expression analyses indicated that the Rhopr-IR transcript is differentially-expressed in all tissues tested, with the highest values observed in the central nervous system (CNS). Treatment of insects with the IR kinase activator BpV(phen), glucose, or porcine insulin resulted in the activation of protein phosphorylation in the fat body, and stimulated the phosphorylation of protein kinase Akt, an evolutionarily conserved key regulator of the intracellular insulin signa...Continue Reading

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Citations

May 28, 2020·Frontiers in Physiology·Umut Toprak
Jan 17, 2021·Insect Biochemistry and Molecular Biology·Jimena LeyriaAngela B Lange
Mar 12, 2021·Ticks and Tick-borne Diseases·Tereza KozelkováPetr Kopáček
Nov 8, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Gleidson Silva-OliveiraKatia C Gondim
Jul 17, 2021·Frontiers in Physiology·Szymon ChowańskiJoanna Pacholska-Bogalska

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Datasets Mentioned

BETA
AAC47458.1

Methods Mentioned

BETA
GTPase
PCR
electrophoresis
Protein Assay

Software Mentioned

TMHMM
NetPhos
MUSCLE
SigmaPlot
Geneious
GraphPad Prism
Molecular Evolutionary Genetics Analysis
Phobius
NetNGlyc
Image Lab

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