A ribonucleic acid-specific antibody produced during autoimmune disease: evidence for nucleotide sequence specificity

European Journal of Immunology
D EilatR Laskov

Abstract

A hybridoma secreting RNA-binding autoantibody has been produced by fusion of spleen cells from autoimmune NZB/NZWF1 mice with drug-resistant IgG2b-producing myeloma cells from BALB/c mice. Studies on the specificity of the purified monoclonal autoantibody revealed: (a) absolute specificity for ribopolynucleotides as compared with deoxyribopolynucleotides; (b) specificity for single-stranded RNA as compared with double-stranded RNA; (c) high affinity for the random copolymer poly(G, C); and (d) preference for the random heteropolymer poly(G, C, U). These studies were complemented by stoichiometric measurements of the antibody-RNA complex and computer analysis of the abundance of various di-, tri- and tetranucleotide sequences in native RNA. Taken together, these data suggest that the antigenic determinant recognized by the monoclonal autoantibody is largely composed of a trinucleotide sequence of single-stranded RNA containing, G, C and U residues.

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Citations

Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D EilatR Laskov
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F TronJ F Bach
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S L Deutscher, J D Keene
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