A rice class-XIV kinesin enters the nucleus in response to cold

Scientific Reports
Xiaolu XuPeter Nick

Abstract

Higher plants possess a large number of kinesins, but lack the minus-end directed dynein motors. However, the kinesin class XIV has strongly expanded, and minus-end directed motors from this class may have taken over functions of cytoplasmic dyneins. In this study, we address the functional aspects of a novel rice homologue of the Arabidopsis class-XIV kinesins ATK1 and ATK5. Since a loss-of-function rice mutant of this kinesin is not viable, the function was studied in tobacco BY-2 as heterologous system. OsDLK-GFP stably expressed in BY-2 cells decorates cortical microtubules, but also can shift into the nucleus of interphase cells. Because of this peculiar localisation, we coined the name Dual Localisation Kinesin (DLK). The nuclear import of this protein is strongly and reversibly promoted in response to cold. During mitosis, OsDLK is repartitioned between spindle and phragmoplast. Motility assays in vitro using show that OsDLK can convey mutual sliding of microtubules and moves at a velocity comparable to other class-XIV kinesins. When tobacco cells overexpressing OsDLK are synchronised, they exhibit a delayed entry into metaphase, while the later phases of mitosis are accelerated. The data are discussed in relation to add...Continue Reading

References

May 24, 1991·Science·A LupasJ Stock
Jan 1, 1994·Cell Motility and the Cytoskeleton·G Q LiuM Cresti
Mar 1, 1995·The Journal of Cell Biology·S Z Wang, R Adler
Jan 7, 1997·Proceedings of the National Academy of Sciences of the United States of America·H SongS A Endow
Feb 1, 1997·Current Opinion in Cell Biology·L A Amos, K Hirose
Feb 5, 1998·The Plant Journal : for Cell and Molecular Biology·J Bowser, A S Reddy
Apr 29, 1998·The Journal of Cell Biology·W O Hancock, J Howard
Jul 31, 1998·Experimental Cell Research·N KudoM Yoshida
Oct 31, 1998·Nature·E P SablinR J Fletterick
Nov 24, 1999·Nature Cell Biology·S A Endow
Mar 15, 2002·International Review of Cytology·Seiichiro Hasezawa, Fumi Kumagai
May 7, 2002·Trends in Plant Science·Mansour KarimiAnn Depicker
Jul 12, 2002·Cell Motility and the Cytoskeleton·Adam I MarcusRichard J Cyr
Apr 11, 2003·Molecular Biology of the Cell·A I MarcusR J Cyr
Mar 11, 2004·Plant Molecular Biology·Margit MengesJames A H Murray
Aug 6, 2004·Plant & Cell Physiology·Carola HolwegPeter Nick
Sep 18, 2004·Plant Physiology·Philip ZimmermannWilhelm Gruissem
Oct 29, 2004·Plant & Cell Physiology·Masayoshi NakamuraTakashi Hashimoto
Jan 22, 2005·Molecular Biology of the Cell·J Christian AmbroseRichard Cyr
Jun 11, 2005·Trends in Cell Biology·Manjari Mazumdar, Tom Misteli
Feb 2, 2006·BMC Genomics·Dale N RichardsonAnireddy S N Reddy
Mar 15, 2006·Trends in Plant Science·Marleen VanstraelenDanny Geelen
May 10, 2006·Current Biology : CB·Sabine MüllerLaurie G Smith
Apr 3, 2007·Biophysical Journal·Dawen CaiEdgar Meyhöfer
Sep 28, 2007·Traffic·Bill Wickstead, Keith Gull
Aug 30, 2008·Plant, Cell & Environment·Andrea KuthanovaZdenek Opatrny
Nov 4, 2008·Current Opinion in Plant Biology·Clive Lloyd, Jordi Chan
May 12, 2009·Nature Cell Biology·Gero FinkStefan Diez
Oct 1, 1963·The Journal of Cell Biology·M C Ledbetter, K R Porter
Jan 1, 2008·Advances in Bioinformatics·Tomas HruzPhilip Zimmermann
Dec 19, 2009·Journal of Experimental Botany·Aleksandra M JovanovićPeter Nick
Apr 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Noriyuki SuetsuguMasamitsu Wada
May 7, 2010·The Plant Journal : for Cell and Molecular Biology·Mu ZhangYihua Zhou
Jun 23, 2010·Journal of Experimental Botany·Nicole FreyPeter Nick
Jun 7, 2011·Biophysical Journal·Felix RuhnowStefan Diez

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Citations

Jan 18, 2020·Journal of Experimental Botany·Sergio Galindo-TrigoLisa M Smith
Sep 17, 2020·Scientific Reports·Priyanka DeveshwarAkhilesh Kumar Tyagi
Apr 7, 2019·Scientific Reports·K SchwarzerováP Nick
Sep 4, 2020·Journal of Experimental Botany·Virginia Natali MiguelRaquel Lia Chan

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Methods Mentioned

BETA
transgenic
Fluorescence
electrophoresis
PCR

Software Mentioned

Zen
FIESTA
COILs
MatLab
AxioVision
Image J
MEGA5

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