A-ring ortho-specific monohydroxylation of daidzein by cytochrome P450s of Nocardia farcinica IFM10152

Biotechnology Journal
Kwon-Young ChoiByung-Gee Kim

Abstract

The bioconversion of the isoflavonoid daidzein using whole cell Nocardia farcinica IFM10152 showed two kinds of major metabolic modifications, i.e. mono-hydroxylation and subsequent O-methylation. The major hydroxylated products of daidzein prior to the O-methylation reaction were 3',4',7-trihydroxyisoflavone (3'-ODI), 4',6,7-trihydroxyisoflavone (6-ODI) and 4',7,8-trihydroxyisoflavone (8-ODI), which are mono-hydroxylated at the ortho position of each hydroxyl group of daidzein. To identify monooxygenases playing a key role in the monohydroxylation of the A-ring of daidzein, all genes of 27 cytochrome P450s from N. farcinica IFM10152 were cloned and transformed into a E. coli BL21 (DE3) host system. By this enzymatic reaction using the mutants and the genome sequence analysis of N. farcinica IFM10152, it was revealed that nfa12130 and nfa33880 P450 genes clustered with their own ferredoxins and ferredoxin reductases (nfa12140+nfa12150 and nfa338870+nfa33860, respectively) are responsible for the hydroxylation of the A-ring of daidzein, and their major reaction products were 6-ODI and 8-ODI, respectively.

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Citations

Apr 8, 2014·International Journal of Molecular Sciences·Te-Sheng Chang
Nov 25, 2011·Enzyme and Microbial Technology·Bishnu Prasad PandeyByung-Gee Kim
Jan 20, 2019·Journal of Industrial Microbiology & Biotechnology·Dipesh DhakalJae Kyung Sohng
Sep 6, 2018·FEMS Microbiology Letters·Pyung-Gang LeeByung-Gee Kim

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