A RING-Type E3 Ubiquitin Ligase, OsGW2, Controls Chlorophyll Content and Dark-Induced Senescence in Rice

International Journal of Molecular Sciences
Kyu-Chan ShimSang-Nag Ahn

Abstract

Leaf senescence is the final stage of plant development. Many internal and external factors affect the senescence process in rice (Oryza sativa L.). In this study, we identified qCC2, a major quantitative trait locus (QTL) for chlorophyll content using a population derived from an interspecific cross between O. sativa (cv. Hwaseong) and Oryza grandiglumis. The O. grandiglumis allele at qCC2 increased chlorophyll content and delayed senescence. GW2 encoding E3 ubiquitin ligase in the qCC2 region was selected as a candidate for qCC2. To determine if GW2 is allelic to qCC2, a gw2-knockout mutant (gw2-ko) was examined using a dark-induced senescence assay. gw2-ko showed delayed leaf senescence in the dark with down-regulated expression of senescence-associated genes (SAGs) and chlorophyll degradation genes (CDGs). The association of the GW2 genotype with the delayed senescence phenotype was confirmed in an F2 population. RNA-seq analysis was conducted to investigate 30-day-old leaf transcriptome dynamics in Hwaseong and a backcross inbred line-CR2002-under dark treatment. This resulted in the identification of genes involved in phytohormone signaling and associated with senescence. These results suggested that transcriptional regul...Continue Reading

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Citations

Aug 23, 2020·International Journal of Molecular Sciences·Kiyosumi Hori, Matthew Shenton
May 1, 2021·International Journal of Molecular Sciences·Sichul Lee, Celine Masclaux-Daubresse
May 1, 2021·Molecular Plant·Jianqin HaoYunhai Li

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Datasets Mentioned

BETA
PRJNA602373

Methods Mentioned

BETA
two-hybrid
pull-down
RNA-seq
PCR
ubiquitination
transgenic

Software Mentioned

Salmon
Limma
FastQC
Voom
agriGO

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