A robust and rapid xenograft model to assess efficacy of chemotherapeutic agents for human acute myeloid leukemia

Blood Cancer Journal
E SalandJ-E Sarry

Abstract

Relevant preclinical mouse models are crucial to screen new therapeutic agents for acute myeloid leukemia (AML). Current in vivo models based on the use of patient samples are not easy to establish and manipulate in the laboratory. Our objective was to develop robust xenograft models of human AML using well-characterized cell lines as a more accessible and faster alternative to those incorporating the use of patient-derived AML cells. Five widely used AML cell lines representing various AML subtypes were transplanted and expanded into highly immunodeficient non-obese diabetic/LtSz-severe combined immunodeficiency IL2Rγc(null) mice (for example, cell line-derived xenografts). We show here that bone marrow sublethal conditioning with busulfan or irradiation has equal efficiency for the xenotransplantation of AML cell lines. Although higher number of injected AML cells did not change tumor engraftment in bone marrow and spleen, it significantly reduced the overall survival in mice for all tested AML cell lines. On the basis of AML cell characteristics, these models also exhibited a broad range of overall mouse survival, engraftment, tissue infiltration and aggressiveness. Thus, we have established a robust, rapid and straightforwa...Continue Reading

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Citations

May 2, 2016·Blood Reviews·Zhiwu DongKewen Zhao
Oct 1, 2016·Biochemical and Biophysical Research Communications·Sruti V Prathivadhi-BhayankaramAndrew E Ekpenyong
Jan 24, 2019·International Journal of Molecular Sciences·Marwa Almosailleakh, Juerg Schwaller
Feb 13, 2019·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Kenneth A FowlerJames M Coghill
Jan 15, 2019·Medicine and Science in Sports and Exercise·Russell EmmonsMichael DE Lisio
Jun 7, 2019·International Journal of Molecular Sciences·Amany AlmarsAbdolrahman S Nateri
Sep 4, 2020·Diagnostics·Anca OnaciuIoana Berindan-Neagoe
Dec 11, 2019·Scientific Reports·María Luz MoralesJoaquín Martínez-López
Aug 28, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Babita KaundalSubhasree Roy Choudhury
Jun 28, 2017·ELife·Xiaochuan ShanUNKNOWN Reproducibility Project: Cancer Biology
Apr 24, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rachel A BurgaCatherine M Bollard
May 27, 2020·ACS Applied Materials & Interfaces·Avinash Chandra KushwahaSubhasree Roy Choudhury

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Methods Mentioned

BETA
xenograft
flow cytometry
dissection
xenografts

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