PMID: 2109601Apr 1, 1990Paper

A role for calcium and protein kinase C in agonist-stimulated adhesion of human neutrophils

The Biochemical Journal
M P DaviesJ E Merritt

Abstract

Stimulated adherence of human neutrophils to plastic and changes in cytosolic free Ca2+ concn. [( Ca2+]i) were measured in the same cell preparations. [Ca2+]i-activation curves were constructed to compare the relation between [Ca2+]i and adhesion in response to ionomycin and formylmethionyl-leucyl-phenylalanine (FMLP). This showed that FMLP-stimulated adhesion required less increase in [Ca2+]i than did ionomycin's effect, a result suggesting that an additional stimulatory component might be involved in the response to FMLP. Protein kinase C activation was a possibility, and activation of protein kinase C with a phorbol ester (PMA) was found to stimulate adhesion with no change in [Ca2+]i. A low concentration of PMA was found to synergize with ionomycin to stimulate a greater adhesion response than with each alone, and the [Ca2+]i-activation curve for ionomycin in the presence of PMA was shifted towards that for FMLP. Thus, synergy between [Ca2+]i and protein kinase C (each of which is sufficient alone) probably explains the stimulatory effects of FMLP on adhesion of neutrophils.

Citations

Jun 7, 2018·European Journal of Clinical Investigation·Roland ImmlerMarkus Sperandio
Jan 25, 2012·Annals of Biomedical Engineering·S D ChaseS I Simon
Jun 24, 2020·Journal of Leukocyte Biology·Mariantonia BraileStefania Loffredo
Feb 6, 2017·Biomedical Engineering Online·Bing HuangJianhua Wu
Feb 7, 2002·Trends in Pharmacological Sciences·Su W LiChris T Poll
Sep 13, 1993·Biochimica Et Biophysica Acta· Novikova IYuL B Margolis

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