A role for chlorophyllide a oxygenase in the regulated import and stabilization of light-harvesting chlorophyll a/b proteins

Proceedings of the National Academy of Sciences of the United States of America
Christiane ReinbotheSteffen Reinbothe

Abstract

The Arabidopsis CAO gene encodes a 52-kDa protein with predicted localization in the plastid compartment. Here, we report that CAO is an intrinsic Rieske iron-sulfur protein of the plastid-envelope inner and thylakoid membranes. Activity measurements revealed that CAO catalyzes chlorophyllide a to chlorophyllide b conversion in vitro and that the enzyme was only slightly active with protochlorophyllide a, the nonreduced precursor of chlorophyllide a. Protein import and organelle fractionation studies identified CAO to be distinct from Ptc52 in the substrate-dependent transport pathway of NADPH:protochlorophyllide oxidoreductase A but instead to be part of a separate translocon complex. This complex was involved in the regulated import and stabilization of the chlorophyllide b-binding light-harvesting proteins Lhcb1 (LHCII) and Lhcb4 (CP29) in chloroplasts. Together, our results provide insights into the plastid subcompartmentalization and evolution of chlorophyll precursor biosynthesis in relation to protein import in higher plants.

References

Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·F Kessler, G Blobel
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·B RathinasabapathiA D Hanson
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·A TanakaK Okada
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·C E EspinedaJ A Brusslan
Mar 29, 2000·The Journal of Biological Chemistry·L L Eggink, J K Hoober
Sep 21, 2000·The Plant Journal : for Cell and Molecular Biology·M GutensohnU I Flügge
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·C ReinbotheS Reinbothe
Apr 21, 2001·Cellular and Molecular Life Sciences : CMLS·J BauerF Kessler
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·H XuW Vermaas
Jul 1, 1995·The Plant Cell·D. Von WettsteinC. G. Kannangara
May 27, 2003·Molecular & Cellular Proteomics : MCP·Myriam FerroNorbert Rolland
Sep 17, 2003·Molecular & Cellular Proteomics : MCP·Ken YamaneHiromu K Mishima
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Adriana PruzinskáStefan Hörtensteiner
Dec 23, 2003·The Plant Cell·Chanhong Kim, Klaus Apel
Feb 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Steffen ReinbotheChristiane Reinbothe
Feb 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Steffen ReinbotheChristiane Reinbothe
Mar 19, 2005·The Plant Journal : for Cell and Molecular Biology·Steffen ReinbotheChristiane Reinbothe
May 24, 2005·Plant Physiology·Amy BaldwinPaul Jarvis
Oct 27, 2005·Molecular & Cellular Proteomics : MCP·Thomas WilhelmSui Huang

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Citations

May 17, 2007·Photosynthesis Research·J Kenneth HooberMin Chen
Jun 15, 2007·Photosynthesis Research·Maryse A BlockNorbert Rolland
Mar 20, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sandra BartschChristiane Reinbothe
Jan 18, 2007·Annual Review of Plant Biology·Ryouichi Tanaka, Ayumi Tanaka
Oct 22, 2013·International Journal of Molecular Sciences·Yi-Hsien LinLi-Fen Huang
Jun 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Vanesa B TognettiNéstor Carrillo
May 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Andreas SchemenewitzSteffen Reinbothe
Jan 5, 2007·Proceedings of the National Academy of Sciences of the United States of America·Katrin PhilipparJürgen Soll
Jun 25, 2014·Antioxidants & Redox Signaling·Peter Geigenberger, Alisdair R Fernie
Aug 17, 2010·European Journal of Cell Biology·Christine V RichterDanja Schünemann
Dec 2, 2008·Trends in Plant Science·Jeferson Gross, Debashish Bhattacharya
Oct 30, 2015·Journal of Plant Physiology·O V Voitsekhovskaja, E V Tyutereva
Sep 12, 2009·The EMBO Journal·Mark T Waters, Jane A Langdale
Nov 30, 2012·Plant, Cell & Environment·Guy Hanke, Paula Mulo
Feb 18, 2015·Biochimica Et Biophysica Acta·Luca Dall'OstoRoberto Bassi
Mar 13, 2017·Photosynthesis Research·Silas Busck MellorPoul Erik Jensen
Sep 17, 2019·PloS One·Franziska PauschScarlett Trimborn
Jun 10, 2020·Plant, Cell & Environment·Marco LandiMassimiliano Tattini
Feb 4, 2012·The Arabidopsis Book·Ryouichi TanakaTatsuru Masuda
Jun 4, 2019·Frontiers in Plant Science·Steffen ReinbotheJohn Gray
Jan 1, 2021·International Journal of Molecular Sciences·Joanna WójtowiczKatarzyna B Gieczewska
Sep 8, 2018·Journal of the American Chemical Society·April L LukowskiAlison R H Narayan
Aug 28, 2021·Biomolecules·Yi-Ting WangJei-Fu Shaw

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