PMID: 9548725May 23, 1998Paper

A role for Dictyostelium racE in cortical tension and cleavage furrow progression

The Journal of Cell Biology
N GeraldA De Lozanne

Abstract

The small GTPase racE is essential for cytokinesis in Dictyostelium. We found that this requirement is restricted to cells grown in suspension. When attached to a substrate, racE null cells form an actomyosin contractile ring and complete cytokinesis normally. Nonetheless, racE null cells fail completely in cytokinesis when in suspension. To understand this conditional requirement for racE, we developed a method to observe cytokinesis in suspension. Using this approach, we found that racE null cells attempt cytokinesis in suspension by forming a contractile ring and cleavage furrow. However, the cells form multiple blebs and fail in cytokinesis by regression of the cleavage furrow. We believe this phenotype is caused by the extremely low level of cortical tension found in racE null cells compared to wild-type cells. The reduced cortical tension of racE null cells is not caused by a decrease in their content of F-actin. Instead, mitotic racE null cells contain abnormal F-actin aggregates. These results suggest that racE is essential for the organization of the cortical cytoskeleton to maintain proper cortical integrity. This function of racE is independent of attachment to a substrate, but can be bypassed by other signaling path...Continue Reading

References

May 1, 1976·Experimental Cell Research·C A Erickson, J P Trinkaus
Aug 1, 1991·Experimental Cell Research·C R Keese, I Giaever
Feb 1, 1990·The Journal of Cell Biology·Y FukuiJ A Spudich
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·I Giaever, C R Keese
Jun 1, 1995·The Journal of Cell Biology·C C Cunningham
Jan 1, 1994·Annual Review of Cell Biology·A Hall
Nov 1, 1993·Zygote : the Biology of Gametes and Early Embryos·I MabuchiS Narumiya
Dec 1, 1995·The Journal of Cell Biology·K D NovakM A Titus
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·S L MooresJ A Spudich
Jun 15, 1996·Microscopy Research and Technique·S M Laster, J M Mackenzie
Sep 20, 1996·The Journal of Biological Chemistry·S KurodaK Kaibuchi
Jan 1, 1996·Cell Motility and the Cytoskeleton·H U Keller, H Bebie
Jan 1, 1997·Current Biology : CB·D N DrechselM Glotzer
Feb 1, 1997·Current Opinion in Cell Biology·N Tapon, A Hall
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C R Keese, I Giaever

❮ Previous
Next ❯

Citations

Mar 31, 1999·Cell Motility and the Cytoskeleton·Y FukuiE L de Hostos
Feb 27, 2001·Cell Motility and the Cytoskeleton·N J GeraldA De Lozanne
Sep 5, 2002·Cell Motility and the Cytoskeleton·Jonathan R ChubbRobert H Insall
Jun 16, 2004·Current Opinion in Cell Biology·Douglas N Robinson, James A Spudich
Apr 9, 2005·Trends in Cell Biology·Elizabeth M ReichlDouglas N Robinson
Jan 20, 2004·Experimental Cell Research·Jae-Seon LeeToru Miki
Mar 31, 2004·Experimental Cell Research·Madhavi AgarwalDenis A Larochelle
Feb 13, 2001·Trends in Genetics : TIG·A Wilkins, R H Insall
Feb 19, 2000·Current Opinion in Cell Biology·G Gerisch, I Weber
Feb 27, 1999·Current Opinion in Cell Biology·J D Sutherland, W Witke
Sep 22, 2001·Current Biology : CB·D N Robinson
May 10, 2000·Trends in Cell Biology·D N Robinson, J A Spudich
Nov 5, 2002·Journal of Molecular Graphics & Modelling·Madhavi AgarwalDenis A Larochelle
Jan 17, 2003·Molecular Biology of the Cell·Jonathan C AbysalhDenis A Larochelle
Jan 6, 2006·Molecular Biology of the Cell·Disha DadkeErica A Golemis
Oct 20, 2006·Molecular Biology of the Cell·Edelyn OcttavianiDouglas N Robinson
Jun 11, 2003·Eukaryotic Cell·Eunkyung LeeDavid A Knecht
May 3, 2000·Cell Structure and Function·T Q UyedaS Yumura
Apr 11, 2002·Cell Structure and Function·H Adachi
Apr 11, 2002·Cell Structure and Function·I Weber
Mar 13, 2014·The Journal of Cell Biology·Evgeny ZatulovskiyRobert R Kay
Nov 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yu WangMiho Iijima
May 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Wendy Zhang, Douglas N Robinson
Mar 8, 2000·The Journal of Cell Biology·S N ProkopenkoH J Bellen
Sep 5, 2002·Cell Motility and the Cytoskeleton·Igor WeberGünther Gerisch
Sep 25, 2003·Cell Motility and the Cytoskeleton·Natalie SchneiderGerard Marriott
Aug 17, 2010·Seminars in Cell & Developmental Biology·Alexandra SurcelDouglas N Robinson
Feb 19, 2015·Biophysical Journal·Begoña Álvarez-GonzálezJuan C Del Álamo
Sep 29, 2015·Scientific Reports·Karthic SwaminathanAngelika A Noegel
Jun 12, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Md Kamruzzaman PramanikShigehiko Yumura
May 18, 2013·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·A Y K Masud RanaShigehiko Yumura
Aug 13, 2013·Biophysical Journal·Inês Mendes PintoRong Li
Oct 21, 2015·Seminars in Cell & Developmental Biology·Vasudha SrivastavaDouglas N Robinson
Oct 19, 2010·Current Biology : CB·Qiongqiong ZhouDouglas N Robinson
Mar 29, 2008·Current Biology : CB·Elizabeth M ReichlDouglas N Robinson
May 10, 2000·Current Biology : CB·I WeberG Gerisch
Jan 10, 2012·Current Biology : CB·Andy LoriaMichael Glotzer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.