A role for Drosophila dFoxO and dFoxO 5'UTR internal ribosomal entry sites during fasting.

PloS One
Eugenia Villa-CuestaMarc Tatar

Abstract

One way animals may cope with nutrient deprivation is to broadly repress translation by inhibiting 5'-cap initiation. However, under these conditions specific proteins remain essential to survival during fasting. Such peptides may be translated through initiation at 5'UTR Internal Ribosome Entry Sites (IRES). Here we show that the Drosophila melanogaster Forkhead box type O (dFoxO) transcription factor is required for adult survival during fasting, and that the 5'UTR of dfoxO has the ability to initiate IRES-mediated translation in cell culture. Previous work has shown that insulin negatively regulates dFoxO through AKT-mediated phosphorylation while dFoxO itself induces transcription of the insulin receptor dInR, which also harbors IRES. Here we report that IRES-mediated translation of both dFoxO and dInR is activated in fasted Drosophila S2 cells at a time when cap-dependent translation is reduced. IRES mediated translation of dFoxO and dInR may be essential to ensure function and sensitivity of the insulin signaling pathway during fasting.

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Citations

Jul 31, 2012·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Binika HadaKyung-Jin Min
Mar 29, 2012·Nature Communications·Yingbiao Ji, Alexei V Tulin
Apr 5, 2015·Cellular and Molecular Life Sciences : CMLS·Sujin ParkJin Won Cho
Apr 27, 2012·Comparative and Functional Genomics·Encarnación Martínez-SalasNoemí Fernández
Jan 11, 2017·Nature Communications·Taru MuranenNada Y Kalaany
Nov 6, 2013·International Journal of Molecular Sciences·Encarnación Martínez-SalasRosa Diaz
Oct 12, 2014·Biology Open·Sabarish Nagarajan, Savraj S Grewal

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Datasets Mentioned

BETA
LD05569

Methods Mentioned

BETA
ubiquitination
acetylation
nuclear translocation
Protein Assay
PCR
Assay
transfection

Software Mentioned

Leica Confocal Lite

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