A role for Drosophila IAP1-mediated caspase inhibition in Rac-dependent cell migration

Cell
Erika R Geisbrecht, Denise J Montell

Abstract

Border cell migration in the Drosophila ovary is a relatively simple and genetically tractable model for studying the conversion of epithelial cells to migratory cells. Like many cell migrations, border cell migration is inhibited by a dominant-negative form of the GTPase Rac. To identify new genes that function in Rac-dependent cell motility, we screened for genes that when overexpressed suppressed the migration defect caused by dominant-negative Rac. Overexpression of the Drosophila inhibitor of apoptosis 1 (DIAP1), which is encoded by the thread (th) gene, suppressed the migration defect. Moreover, loss-of-function mutations in th caused migration defects but, surprisingly, did not cause apoptosis. Mutations affecting the Dark protein, an activator of the upstream caspase Dronc, also rescued RacN17 migration defects. These results indicate an apoptosis-independent role for DIAP1-mediated Dronc inhibition in Rac-mediated cell motility.

References

Feb 9, 1996·Cell·D A Lauffenburger, A F Horwitz
May 1, 1996·The Journal of Cell Biology·A M Murphy, D J Montell
Dec 22, 1998·Mechanisms of Development·P Rørth
Feb 27, 1999·Current Opinion in Cell Biology·P Aspenström
Mar 19, 1999·Progress in Molecular and Subcellular Biology·J Settleman
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·A G UrenT Lithgow
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·H J Yoon, J Carbon
Feb 17, 2000·The EMBO Journal·P MeierG I Evan
May 19, 2000·The Biochemical Journal·A L Bishop, A Hall
Aug 23, 2000·The Journal of Cell Biology·A Ridley
Aug 25, 2000·Molecular and Cellular Biology·S Yayoshi-YamamotoT Watanabe
Nov 18, 2000·Experimental Cell Research·A A SchmitzL Van Aelst
Aug 18, 2001·The Journal of Biological Chemistry·D A LinsemanK A Heidenreich
Dec 12, 2001·Nature Cell Biology·Isabelle SagotDavid Pellman
Jan 5, 2002·Experimental Cell Research·Julia C Feldner, Burkhard H Brandt
Mar 29, 2002·Nature·Satoko Hakeda-SuzukiBarry J Dickson
Mar 29, 2002·Nature·Julian NgLiqun Luo
May 22, 2002·Nature Cell Biology·Soon Ji YooBruce A Hay
Jul 23, 2002·Nature Cell Biology·Erika R Geisbrecht, Denise J Montell
Jul 23, 2002·Nature Cell Biology·Isabelle SagotDavid Pellman
Dec 13, 2002·Nature·Sandrine Etienne-Manneville, Alan Hall
Jan 4, 2003·Nature Reviews. Molecular Cell Biology·Denise J Montell
Jan 15, 2003·Biochemistry·Martin PringSally H Zigmond
Apr 1, 2003·Journal of Cell Science·Sreenivas KokaJennifer J Westendorf
Aug 5, 2003·Molecular and Cellular Biology·Baolin ZhangEmily Shacter
Sep 17, 2003·The Journal of Cell Biology·Stephen L RogersRonald D Vale
Oct 14, 2003·Biochemical Pharmacology·Christian Schwerk, Klaus Schulze-Osthoff
Dec 12, 2003·Development·Damali N Martin, Eric H Baehrecke

❮ Previous
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Citations

Feb 13, 2009·Apoptosis : an International Journal on Programmed Cell Death·Dawn M CooperCarl Lowenberger
Apr 18, 2009·Apoptosis : an International Journal on Programmed Cell Death·Yael Feinstein-Rotkopf, Eli Arama
Jun 6, 2009·Apoptosis : an International Journal on Programmed Cell Death·Mariam Orme, Pascal Meier
Sep 7, 2013·Cell Death & Disease·T K Oberoi-KhanujaK Rajalingam
May 20, 2005·Nature·Vanessa DepaepePierre Vanderhaeghen
Mar 3, 2007·Nature Cell Biology·Maryse Bailly
Oct 20, 2007·Nature Protocols·Mohit PrasadDenise J Montell
May 3, 2005·Nature Reviews. Cancer·Honami Naora, Denise J Montell
Dec 2, 2004·Nature Reviews. Genetics·Bruce A HayMing Guo
Sep 25, 2012·Nature Reviews. Molecular Cell Biology·Denise J MontellMichelle Starz-Gaiano
Dec 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Gloria AssakerGregory Emery
Jun 3, 2010·Cold Spring Harbor Perspectives in Biology·Pierre Vanderhaeghen, Hwai-Jong Cheng
Mar 17, 2011·Genes & Development·Ji Hoon KimDenise J Montell
Aug 6, 2013·Genes & Development·Alejandro D'BrotJohn M Abrams
Feb 23, 2011·Development, Growth & Differentiation·Erina Kuranaga
Oct 14, 2004·Science's STKE : Signal Transduction Knowledge Environment·Sharad Kumar
Jul 18, 2006·Annual Review of Cell and Developmental Biology·Bruce A Hay, Ming Guo
Sep 15, 2012·Journal of Cell Science·Amanda R BrockMichael J Galko
Jun 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Marc FurriolsJordi Casanova
Jul 14, 2010·Proceedings of the National Academy of Sciences of the United States of America·Robin E C LeeLynn A Megeney
Apr 2, 2014·Médecine sciences : M/S·Laurence DubrezJean Berthelet
Mar 31, 2005·Seminars in Cell & Developmental Biology·Peter CashioAndreas Bergmann
Mar 4, 2011·Neurochemical Research·Masanori ItohToshiyuki Nakagawa
Oct 4, 2005·Médecine sciences : M/S·Vanessa Depaepe, Pierre Vanderhaeghen
Oct 21, 2006·Cell Death and Differentiation·M LamkanfiP Vandenabeele
Oct 14, 2009·The Journal of Cell Biology·Akiko KotoMasayuki Miura
Feb 9, 2011·The Journal of Cell Biology·Minna PoukkulaPernille Rørth
Oct 30, 2015·Scandinavian Journal of Gastroenterology·Jakob B Seidelin

❮ Previous
Next ❯

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