PMID: 18719370Aug 23, 2008Paper

A role for Drosophila Polo protein in chromosome resolution and segregation during mitosis

Cell Cycle
Susana Godinho, Alvaro A Tavares

Abstract

Correct chromosome structure is essential to ensure faithful segregation during mitosis. Chromosome condensation occurs at the same time as cohesion is released from the arms of the sister chromatids. It is not until metaphase-anaphase transition that chromosomes lose cohesion completely, by proteolysis of the component of the cohesin complex Scc1 (Sister chromatid cohesion 1). It has been shown in vertebrates that the Polo-like kinase, Plk1, is important for this process by inducing the destabilization of Scc1 from the chromosome arms. It is still unclear if this process is conserved in other high eukaryotes, namely in Drosophila. Here we analysed the consequences over chromosome resolution of the downregulation of Drosophila Polo, both by mutant analysis and by RNAi-depletion in S2 cells. We show that the depletion of Polo results in a strong prometa/metaphase arrest with the spindle checkpoint activated in response to lack of tension. In addition, the checkpoint protein ROD fails to stream over the kinetochore microtubules in the lack of Polo activity. We also show that loss of Polo causes strong defects in chromosome resolution, a phenotype we partially rescued by depleting Scc1. Importantly, we show Scc1 fails to accumulat...Continue Reading

Citations

Apr 16, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Guojun ZhangZhuogang Liu
Jun 10, 2011·Cell Cycle·Guillermo de CárcerMarcos Malumbres
Jul 16, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Paulina WachowiczGuillermo de Cárcer
Nov 25, 2017·Expert Opinion on Therapeutic Targets·Radhika Radha ManiswamiSooriya Kumar Jegatheesan
Jan 24, 2020·The Journal of Cell Biology·Marie Anne O'Donnell

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