A role for gangliosides and β1-integrin in the motility of olfactory ensheathing glia.

Journal of Anatomy
A Santos-SilvaL A Cavalcante

Abstract

Olfactory ensheathing glia (OEG) are found in the olfactory mucosa, nerve and bulb, and provide in vivo ensheathment for the unmyelinated olfactory axons within the central and peripheral nervous system domains. OEG cells are able to migrate long distances within the neuropil of the central nervous system. Because gangliosides such as 9-O-acetyl GD3 have crucial regulatory roles in neuronal migration during development, we analyzed whether OEG in organotypical cultures are revealed by anti-9-O-acetyl GD3 and/or gangliosides are recognized by the A2B5 antibody (G-A2B5), and whether these gangliosides are involved in OEG migration. Our results showed that all OEG migrating out of a section of olfactory bulb onto a laminin substrate bound to the 9-O-acetyl GD3 and A2B5 antibodies, and that 2',3'-cyclic nucleotide phosphodiesterase (CNPase) colocalized with 9-O-acetyl GD3 and with G-A2B5. Additionally, we showed that the immune blockade of 9-O-acetyl GD3 or G-A2B5 reduced the migration of OEG on laminin, and that 9-O-acetyl GD3 and G-A2B5 colocalized with the β1-integrin subunit. We also confirmed the phenotype of in-vitro-grown OEG cells derived from adult rats, showing that they express CNPase, and also α-smooth muscle actin, whi...Continue Reading

References

Oct 1, 1984·The Anatomical Record·J R Doucette
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·T YamashitaR L Proia
Mar 29, 2001·Molecular and Cellular Neurosciences·M F SantiagoR Mendez-Otero
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sen-itiroh Hakomori Si
Mar 6, 2003·The Anatomical Record. Part B, the New Anatomist·J G BoydR Doucette
May 29, 2003·Biochimie·S BirkléJ Aubry
Sep 16, 2003·Journal of Neurobiology·Erika M A NegreirosRosalia Mendez-Otero
Mar 9, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Richard BelvindrahUlrich Müller
May 12, 2007·The Journal of Comparative Neurology·Ali JahedMichael D Kawaja
Apr 22, 2009·Journal of Neuroscience Research·Edina SilajdzićSusan C Barnett
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Roger A KroesJoseph R Moskal
Sep 3, 2011·Cold Spring Harbor Perspectives in Biology·Anna Huttenlocher, Alan Rick Horwitz
Dec 17, 2011·Journal of Biomedicine & Biotechnology·Christine RadtkeJeffery D Kocsis
May 27, 2015·Nutrients·Kate PalmanoPaul McJarrow
Jun 5, 2016·Journal of Molecular Biology·Ronald L Schnaar
Nov 23, 2016·The Journal of Cell Biology·Zhiqi SunReinhard Fässler

❮ Previous
Next ❯

Citations

May 18, 2020·Cellular and Molecular Neurobiology·Fernanda S O CamposLeny A Cavalcante

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Actin, Myosin & Cell Movement

Contractile forces generated by the actin-myosin cytoskeleton are critical for morphogenesis, but the cellular and molecular mechanisms of contraction have been elusive for many cell shape changes and movements. Here is the latest research on the roles of actin and myosin in cell movement.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.