Abstract
A Candida glabrata cadmium-sensitive mutant partially defective in glutathione production and exhibiting a complete absence of phytochelatins was used to clone a gene required for Cd tolerance. Transformation of the Cd-sensitive mutant with a genomic library from the wild-type C. glabrata led to the cloning of a gene that restored Cd tolerance and formation of Cd-glutathione and Cd-phytochelatin complexes. The cloned gene showed high levels of nucleic acid and protein sequence homology to the HEM2 genes, encoding porphobilinogen synthases, from several sources. It was shown that the C, glabrata Cd-sensitive mutant indeed exhibited a significant reduction in porphobilinogen synthase levels. The cloned C. glabrata gene complemented a hem2 mutant of Saccharomyces cerevisiae and restored porphobilinogen synthase activity in the mutant. The Cd sensitive mutant predictably showed decreased levels of sulfite reductase that requires siroheme, a metabolite produced in the heme biosynthetic pathway. The addition of cysteine, but not methionine, increased glutathione levels and Cd tolerance of both the wild-type and the mutant strain. However, addition of hemin chloride and methionine together restored Cd tolerance indicating that heme wa...Continue Reading
References
Nov 11, 1992·Journal of Inorganic Biochemistry·J BarbasD R Winge
Dec 1, 1992·Molecular and Cellular Biology·D M SpeiserD W Ow
Apr 1, 1992·Gene·R K MehraD R Winge
Jan 1, 1991·Journal of Cellular Biochemistry·R K Mehra, D R Winge
Feb 1, 1991·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Y HayashiT Goto
Jul 1, 1990·Yeast·L M BorralhoJ R Mattoon
May 1, 1989·Genetics·R S Sikorski, P Hieter
Sep 1, 1987·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R K SinghalA Meister
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R K MehraD R Winge
Sep 1, 1986·Yeast·J E HillA Tzagoloff
Jan 1, 1985·Methods in Enzymology·M E Anderson
Feb 1, 1995·Applied and Environmental Microbiology·V JiranekP A Henschke
Apr 1, 1995·Journal of Bioenergetics and Biomembranes·E K Jaffe
Apr 1, 1995·Plant Physiology·R HowdenC S Cobbett
Apr 1, 1995·Plant Physiology·R HowdenC S Cobbett
Aug 1, 1994·FEMS Microbiology Reviews·A Coblenz, K Wolf
May 16, 1994·Biochemical and Biophysical Research Communications·R K MehraT C Hunter
Oct 1, 1993·The Biochemical Journal·N J RobinsonP J Jackson
Sep 1, 1993·Journal of Bacteriology·H CherestY Surdin-Kerjan
Dec 1, 1995·Plant Physiology·W E Rauser
Feb 15, 1996·The Biochemical Journal·R K MehraP Mulchandani
May 1, 1996·Current Genetics·C M GrantI W Dawes
Nov 7, 1996·Gene·M H Zenk
Jan 7, 1997·Proceedings of the National Academy of Sciences of the United States of America·Z S LiP A Rea
Jan 1, 1997·Annual Review of Nutrition·R A Goyer
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E GrillM H Zenk
Citations
Feb 19, 2009·Applied Biochemistry and Biotechnology·Rama Pal, J P N Rai
Sep 12, 2002·Annual Review of Plant Biology·Christopher Cobbett, Peter Goldsbrough
May 20, 2000·Chemosphere·A G KhanW J Hayes
May 12, 2020·Molecular Ecology Resources·Li BianZhishu Lin
Oct 26, 1999·Endocrine Reviews·V Giguère
Jul 1, 2020·Molecular Ecology Resources·Fenghui LiSiqing Chen
Oct 29, 2000·Journal of Inorganic Biochemistry·K N MacleanJ P Kraus