A role for increased lutropin/choriogonadotropin receptor (LHR) gene transcription in the follitropin-stimulated induction of the LHR in granulosa cells

Molecular Endocrinology
H Shi, D L Segaloff

Abstract

Follitropin (FSH) has been shown in previous studies to stimulate the induction of the LH/CG receptor (LHR) and LHR mRNA in the granulosa cells of diethylstilbesterol-primed immature rats. The present studies were undertaken to identify the mechanisms underlying the hormone-dependent induction of the LHR in rat granulosa cells. The effect of FSH on LHR mRNA stability was determined by measuring the decay of LHR mRNA after removal of FSH under conditions where transcription was inhibited. Under these conditions, readdition of FSH had little effect on mRNA stability. However, inhibitors of transcription themselves were found to have a marked effect on stabilizing the LHR mRNA, thus potentially masking an effect of FSH. These results suggest that there is a labile destabilizing factor that constitutively degrades LHR mRNA. At present, it cannot be ascertained whether FSH has any effect on this destabilizing factor. Transcriptional activity of the LHR gene was examined using nuclear run-on assays. It was found that 1) in the absence of FSH, LHR-binding activity and mRNA levels were negligible, but the LHR gene was transcriptionally active in granulosa cells of immature rats; 2) incubations of granulosa cells with FSH or 8-bromo-cAM...Continue Reading

Citations

Jul 17, 2013·BMC Systems Biology·Dagmar Iber, Christian De Geyter
Jun 23, 2001·Molecular and Cellular Endocrinology·S G Hillier
Aug 29, 2001·Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao·C Zhu, H Tian
Apr 11, 2008·Endocrine·Rosalba Escamilla-HernandezAnthony J Zeleznik
Feb 3, 1997·FEBS Letters·H KishiK Miyamoto
Jan 26, 2017·Reproduction in Domestic Animals = Zuchthygiene·J ZahmelB C Braun
Oct 1, 2010·Human Molecular Genetics·Enrica BianchiClaudio Sette

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