Abstract
The gastrointestinal (GI) microbiome has emerged as a potential regulator of metabolism. However, the precise mechanisms of how microorganisms may influence physiology remain largely unknown. Interestingly, GI microorganisms, including methanogens, are localized within the same regions as the glucagon-like peptide-1 (GLP-1) secreting L cells. GLP-1 plays key roles appetite and glucose regulation. Furthermore, both methane and GLP-1 levels are altered in obese humans with metabolic disease. We predict that high-fat diet-induced obesity alters the abundance of GI methanogens and that methane may play a role in the GLP-1 secretory response from the L cell. To demonstrate this, GLP-1 secretion response and faecal methanogens were examined in mice given a high-fat diet for 14 weeks. In addition, the direct effect of methane on GLP-1 secretion was assessed in two L-cell models (NCI-H716 and GLUTag). High-fat diet caused a significant increase in both GLP-1 secretion and faecal methanogen content. There was a direct correlation between GLP-1 secretion response and faecal methanogen levels. In L cells, methane stimulated GLP-1 secretion and enhanced intracellular cAMP content. These results indicate that alterations in the methanogen c...Continue Reading
References
Jan 1, 1985·Gut·L F McKayW G Brydon
Dec 2, 2008·Nature·Peter J TurnbaughJeffrey I Gordon
Mar 8, 2011·Microbial Biotechnology·Takashi Narihiro, Yuji Sekiguchi
Jul 15, 2011·The ISME Journal·Gerardo M NavaH Rex Gaskins
Dec 23, 2011·Diabetes·Gwen TolhurstFiona M Gribble
Sep 27, 2012·Medical Gas Research·Wenwu LiuXuejun Sun
Oct 3, 2012·Nature Reviews. Gastroenterology & Hepatology·Charlotte X Dong, Patricia L Brubaker
Mar 28, 2013·The Journal of Clinical Endocrinology and Metabolism·R MathurM Pimentel
Sep 7, 2013·Science·Vanessa K RidauraJeffrey I Gordon
Feb 14, 2015·Diabetes·Kristine FærchMarit E Jørgensen
Apr 2, 2015·The British Journal of Nutrition·Shingo NakajimaHiroshi Hara
Aug 14, 2015·Endocrinology·Jeffrey GagnonPatricia L Brubaker
Sep 13, 2015·Biochemical and Biophysical Research Communications·Jiangchun WuLin Liu
Jan 9, 2016·The Journal of Clinical Endocrinology and Metabolism·Hannes ManellPeter Bergsten
Feb 3, 2016·Obesity·Ruchi MathurMark Pimentel
Citations
Sep 7, 2019·Journal of Zhejiang University. Science. B·Er-Teng JiaQin-Yu Ge
Jul 22, 2020·Nature Reviews. Microbiology·Guillaume BorrelChristine Moissl-Eichinger
Oct 20, 2018·Scientific Reports·José M S Fernández-CallejaEvert M van Schothorst
Dec 14, 2019·Journal of Breath Research·Naoki OzatoShigeyuki Nakaji
Jun 15, 2019·International Journal of Molecular Sciences·Ziguo SongChao Sun
May 19, 2021·The ISME Journal·Yueqiong NiGianni Panagiotou