A Role for MYC in Lithium-Stimulated Repair of the Colonic Epithelium After DSS-Induced Damage in Mice

Digestive Diseases and Sciences
Wesley M Raup-KonsavageGregory S Yochum

Abstract

Chronic inflammation disrupts the colonic epithelial layer in patients afflicted by ulcerative colitis (UC). The use of inhibitors of glycogen synthase kinase three beta (GSK3β) has proven efficacious to mitigate disease symptoms in rodent models of UC by reducing the pro-inflammatory response. Less is known about whether these inhibitors promote colonic regeneration by stimulating proliferation of colonic epithelial cells. We investigated whether delivery of the GSK3β inhibitor, lithium chloride (LiCl), during the recovery period from acute DSS-induced colitis in mice promoted colonic regeneration and ameliorated disease symptoms. We also tested whether the c-MYC transcription factor (MYC) was involved in this response. Acute colitis was induced by administration of 2.5 % dextran sodium sulfate (DSS) to wild-type C57BL/6 mice for 5 days. During the recovery period, mice received a daily intraperitoneal (IP) injection of LiCl or 1X PBS as a control. Mice were weighed, colon lengths measured, disease activity index (DAI) scores were assessed, and histological analyses were performed on colonic sections. We analyzed transcripts and proteins in purified preparations of the colonic epithelium. We delivered the MYC inhibitor 10058-F...Continue Reading

References

Apr 1, 1987·The Journal of Pathology·P J CiclitiraG Evan
Feb 1, 1972·JAMA : the Journal of the American Medical Association·S Zisook
Apr 1, 1996·Digestive Diseases and Sciences·R J AlexanderR F Raicht
Aug 1, 1997·Digestive Diseases and Sciences·W E RoedigerW Babidge
Sep 4, 1998·Science·T C HeK W Kinzler
Mar 17, 1999·Frontiers in Bioscience : a Journal and Virtual Library·S M Karam
Oct 18, 2003·The Journal of Biological Chemistry·Mark A GregoryStephen R Hann
Jun 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Bruno Amati
Jan 8, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Silvia MelgarErik Michaëlsson
Nov 30, 2005·British Journal of Pharmacology·Brendan J R WhittleChristoph Thiemermann
Aug 15, 2006·Seminars in Cancer Biology·Chi V DangFeng Li
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Gregory S YochumRichard H Goodman
Mar 23, 2007·Nature·Owen J SansomAlan R Clarke
Apr 5, 2007·Nature Protocols·Stefan WirtzMarkus F Neurath
Sep 11, 2007·Advanced Drug Delivery Reviews·Stefan Wirtz, Markus F Neurath
Sep 19, 2007·Molecular Cancer Therapeutics·Huabo WangEdward V Prochownik
Jun 10, 2008·Nature Genetics·Eugenio Sangiorgi, Mario R Capecchi
Dec 17, 2008·Digestive Diseases and Sciences·Ali DaneshmandAhamd Reza Dehpour
Mar 24, 2009·Nature Reviews. Molecular Cell Biology·Christian MosimannKonrad Basler
May 13, 2009·Journal of the American Chemical Society·Dalia I HammoudehSteven J Metallo
Oct 10, 2009·The EMBO Journal·Tokameh MahmoudiHans Clevers
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Gregory S YochumRichard H Goodman
May 13, 2010·Nucleic Acids Research·Daniel BottomlyGregory S Yochum
Sep 18, 2010·Inflammatory Bowel Diseases·Claudia HofmannFlorian Obermeier
Nov 30, 2010·Cell Stem Cell·Nick BarkerHans Clevers
Dec 15, 2010·Nature Reviews. Immunology·Maya Saleh, Giorgio Trinchieri
Oct 7, 2011·American Journal of Physiology. Cell Physiology·Lan LiuJian-Ying Wang
Oct 8, 2011·Experimental Cell Research·Taeko K NoahNoah F Shroyer
Nov 4, 2011·The New England Journal of Medicine·Silvio Danese, Claudio Fiocchi
Nov 15, 2011·Science·Norifumi TakedaJonathan A Epstein
Mar 6, 2012·Nature Cell Biology·Vivian W Y WongKim B Jensen
Jun 12, 2012·Cell·Hans Clevers, Roel Nusse
Jul 25, 2012·Molecular and Cellular Biology·Wesley M KonsavageGregory S Yochum
Aug 24, 2012·Lancet·Ingrid OrdásWilliam J Sandborn
May 4, 2013·Digestive Diseases and Sciences·Wesley M KonsavageGregory S Yochum
Jun 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Hanna ZirathMarie Arsenian Henriksson
Feb 26, 2014·Molecular and Cellular Biology·Wesley M Konsavage, Gregory S Yochum

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Citations

Nov 2, 2015·Digestive Diseases and Sciences·Mark R Frey
Aug 14, 2018·Annals of the New York Academy of Sciences·Di ChenD Rick Sumner
Jul 28, 2020·Journal of Clinical Immunology·Alma ZivDror S Shouval
Sep 28, 2016·World Journal of Gastroenterology : WJG·Ferenc SiposGyörgyi Műzes
Jan 24, 2019·Cannabis and Cannabinoid Research·Wesley M Raup-KonsavageKent E Vrana
Oct 9, 2019·Scientific Reports·Melanie A EshelmanGregory S Yochum
Mar 12, 2021·Journal of Dental Research·J ZhuX Xu

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