A role for nucleoporin FG repeat domains in export of human immunodeficiency virus type 1 Rev protein and RNA from the nucleus.

Molecular and Cellular Biology
Françoise StutzM Rosbash

Abstract

The human immunodeficiency virus type 1 Rev protein contains a nuclear export signal (NES) that is required for Rev-mediated RNA export in mammals as well as in the yeast Saccharomyces cerevisiae. The Rev NES has been shown to specifically interact with a human (hRIP/RAB1) and a yeast (yRip1p) protein in the two-hybrid assay. Both of these interacting proteins are related to FG nucleoporins on the basis of the presence of typical repeat motifs. This paper shows that Rev is able to interact with multiple FG repeat-containing nucleoporins from both S. cerevisiae and mammals; moreover, the ability of Rev NES mutants to interact with these FG nucleoporins parallels the ability of the mutants to promote RNA export in yeast and mammalian cells. The data also show that, after Xenopus oocyte nuclear injection, several FG nucleoporin repeat domains inhibit the export of both Rev protein and U small nuclear RNAs, suggesting that these nucleoporins participate in Rev-mediated and cellular RNA export. Interestingly, not all FG nucleoporin repeat domains produced the same pattern of RNA export inhibition. The results suggest that Rev and cellular mediators of RNA export can interact with multiple components of the nuclear pore complex durin...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·S R WenteG Blobel
Sep 1, 1991·Trends in Biochemical Sciences·B R Cullen, M H Malim
Dec 1, 1990·Molecular and Cellular Biology·M Ivey-Hoyle, M Rosenberg
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Mar 1, 1994·The Journal of Cell Biology·A JarmolowskiI W Mattaj
Apr 21, 1995·Cell·E Izaurralde, I W Mattaj
Jan 1, 1995·Annual Review of Biochemistry·L I Davis
Aug 24, 1995·Nature·E IzaurraldeI W Mattaj
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·R L Finley, R Brent
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·A RaduM S Moore
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·D KraemerA Radu
Mar 15, 1996·Science·D Görlich, I W Mattaj
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·R A FridellB R Cullen
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·R A FridellB R Cullen
Oct 1, 1994·Trends in Cell Biology·M P Rout, S R Wente

❮ Previous
Next ❯

Citations

Aug 30, 2011·Virus Research·Brian M FriedrichMonique R Ferguson
Jul 29, 2011·Wiley Interdisciplinary Reviews. RNA·Nadeem Siddiqui, Katherine L B Borden
Jul 11, 2012·Journal of Biomedicine & Biotechnology·Marie-Line Andréola, Simon Litvak
Dec 2, 1999·Experimental Cell Research·E SantoliniP P Di Fiore
Feb 26, 1998·Cell·M OhnoI W Mattaj
Jul 31, 1998·Experimental Cell Research·N KudoM Yoshida
May 1, 1997·Chemistry & Biology·T J Hope
Nov 21, 2012·PloS One·Mohamed Ali JarbouiVirginie W Gautier
Aug 6, 2010·PLoS Computational Biology·Jamie I MacPhersonDavid L Robertson
Jan 19, 1999·Annual Review of Microbiology·V W Pollard, M H Malim
Apr 23, 2014·Nucleic Acids Research·Ichiro TaniguchiMutsuhito Ohno
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·A E PasquinelliJ E Dahlberg
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·O SchatzJ Hauber
Jun 8, 1999·The Journal of Biological Chemistry·G FarjotI Mikaélian
Jan 30, 2008·Current Protocols in Cell Biology·D Duricka, K S Ullman
Oct 31, 2001·The Journal of Cell Biology·S VasuD J Forbes
Oct 6, 1997·Science·B Ossareh-NazariC Dargemont
May 29, 1999·The Journal of Biological Chemistry·M Floer, G Blobel
Sep 14, 2002·The Journal of Biological Chemistry·Erwann Le RouzicSerge Benichou
May 11, 2000·Molecular and Cellular Biology·I E VainbergM Rosbash
Aug 24, 1999·Molecular and Cellular Biology·P AskjaerM Fornerod
Jul 17, 1998·Medicinal Research Reviews·D A JansS Huebner
Jul 20, 2001·Journal of Virology·J ParagasP Palese
Jul 1, 1997·Biological Chemistry·G J ArtsI W Mattaj
Feb 3, 2011·Immunopharmacology and Immunotoxicology·Maria Antonietta PanaroAntonia Cianciulli
Apr 10, 1999·Journal of Virology·T L SymensmaA D Ellington

❮ Previous
Next ❯

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