PMID: 6539129May 22, 1984Paper

A role for ornithine in the regulation of putrescine accumulation and ornithine decarboxylase activity in Reuber H35 hepatoma cells

Biochimica Et Biophysica Acta
V S Wu, C V Byus

Abstract

We investigated the ability of intracellular ornithine to alter both the biosynthesis of putrescine and the activity of ornithine decarboxylase in Reuber H35 hepatoma cells in culture incubated with 12-O- tetrade - canoylphorbol 13-acetate (TPA). In confluent cultures of H35 cells, the addition of TPA (1.6 microM) caused the activity of ornithine decarboxylase to increase by more than 100-fold within 4 h. When exogenous ornithine (0.1-1.0 mM) was added to the culture medium with TPA, a marked dose-dependent increase in the production of putrescine was observed. The activity of ornithine decarboxylase in the same cultures incubated with ornithine decreased in a similar dose-dependent manner. The addition of arginine (0.1-1.0 mM) (but not lysine or histidine) to the H35 cells in culture concomitant with TPA also led to a relative increase in putrescine biosynthesis and a decrease in ornithine decarboxylase activity compared to cultures not receiving the amino acids. A similar response to exogenous ornithine and TPA was observed in a series of less confluent rapidly growing cultures which were in culture for a shorter period of time. The confluent cultures possessed a basal level of arginase (55 units/mg protein) which increased a...Continue Reading

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Citations

Jan 1, 1989·The International Journal of Biochemistry·N SeilerB Knödgen
Mar 1, 1995·The International Journal of Biochemistry & Cell Biology·M Manteuffel-CymborowskaB Grzelakowska-Sztabert
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M TakigawaF Suzuki
Feb 15, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·X HanB A Stanley

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