A role for Rho kinase in vascular contraction evoked by sodium fluoride

Biochemical and Biophysical Research Communications
Su Bun JeonIn Kyeom Kim

Abstract

Agonist and depolarization-induced vascular smooth muscle contractions involve the activation of Rho-kinase pathway. However, there are no reports addressing the question whether this pathway is involved in NaF-induced vascular contractions. We hypothesized that Rho-kinase plays a role in vascular contraction evoked by sodium fluoride in rat aortae. In both physiological salt solution and calcium-free solution with 2 mM EGTA, cumulative addition of NaF increased vascular tension in concentration-dependent manners. Effects of Rho-kinase inhibitor (Y27632) on phosphorylation of myosin light chain (MLC20) and myosin targeting subunit (MYPT1(Thr696)) of myosin light chain phosphatase as well as NaF-induced contractions were determined using isolated tissue and the Western blot experiments. Y27632 inhibited NaF-induced contractions in a concentration-dependent manner. NaF increased phosphorylation of MLC20 and MYPT1(Thr696), which were also inhibited by Y27632. However, MLCK inhibitor (ML-7) or PKC inhibitor (Ro31-8220) did not inhibit the NaF-induced contraction. These results indicate that activation of Rho-kinase and the subsequent phosphorylation of MYPT1(Thr696) play important roles in NaF-induced contraction of rat aortae.

References

Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T KitazawaA P Somlyo
Jan 1, 1984·The Journal of Clinical Investigation·A G Gilman
Nov 17, 1994·Nature·A P Somlyo, A V Somlyo
Mar 1, 1996·The Journal of Biological Chemistry·K IchikawaD J Hartshorne
Aug 23, 1996·The Journal of Biological Chemistry·M AmanoK Kaibuchi
Jun 23, 1998·Journal of Muscle Research and Cell Motility·D J HartshorneF Erdödi
Jun 26, 1998·Science·T KozasaP C Sternweis
Jan 15, 1999·Acta Physiologica Scandinavica·A P Somlyo, A V Somlyo
Dec 22, 1999·The Journal of Biological Chemistry·J FengT Nakano
Aug 6, 2000·Circulation Research·A GohlaS Offermanns
Apr 27, 2002·The Journal of Biological Chemistry·Meredith A BormanTimothy A J Haystead
Mar 5, 2004·Naunyn-Schmiedeberg's Archives of Pharmacology·In Kyeom KimChoong-Young Kim
May 6, 2004·Molecular and Cellular Biochemistry·Masaaki ItoDavid J Hartshorne
Oct 29, 2004·Cell Biochemistry and Biophysics·Mustafa ArkEmel Songu-Mize
Sep 22, 2005·Clinical and Experimental Pharmacology & Physiology·Cleber E TeixeiraR Clinton Webb

❮ Previous
Next ❯

Citations

May 11, 2007·Archives of Pharmacal Research·Hyun Dong JeUy Dong Sohn
Mar 20, 2009·Naunyn-Schmiedeberg's Archives of Pharmacology·Enyue YangIn Kyeom Kim
Oct 14, 2011·Naunyn-Schmiedeberg's Archives of Pharmacology·Hye Young KimIn Kyeom Kim
May 25, 2010·Pflügers Archiv : European journal of physiology·Hiroyasu SakaiMiwa Misawa
Mar 12, 2009·Molecules and Cells·Hyun Dong Je, Uy Dong Sohn
Nov 7, 2008·Clinical and Experimental Pharmacology & Physiology·Inji BaekIn Kyeom Kim
May 23, 2015·Biomolecules & Therapeutics·Hyun Dong JeHyen-Oh La
May 1, 2010·Environmental Toxicology and Pharmacology·Enyue YangIn Kyeom Kim
Jul 26, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Yun Hwan Kang, Heung Mook Shin
Oct 23, 2010·Journal of Ethnopharmacology·Young Mi SeokIn Kyeom Kim
Feb 17, 2017·Biomolecules & Therapeutics·Yoon Hee ChungHyen-Oh La
Feb 2, 2018·Biomolecules & Therapeutics·Young Sil MinUy Dong Sohn
Feb 27, 2013·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Hyun Dong JeJi Hoon Jeong
Jan 26, 2016·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Hyun Dong Je, Ji Hoon Jeong
Sep 21, 2007·Clinical and Experimental Pharmacology & Physiology·Su Bun JeonIn Kyeom Kim
Jan 31, 2019·Basic & Clinical Pharmacology & Toxicology·Yuki KaiHiroyasu Sakai
Nov 4, 2009·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Inji BaekIn Kyeom Kim
Mar 12, 2010·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Enyue YangIn Kyeom Kim
Jan 18, 2013·American Journal of Physiology. Cell Physiology·Trent ButlerEng-Cheng Chan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.