A role for Saccharomyces cerevisiae Chk1p in the response to replication blocks

Molecular Biology of the Cell
Kaila L SchollaertY Sanchez

Abstract

Replication blocks and DNA damage incurred during S phase activate the S-phase and intra-S-phase checkpoint responses, respectively, regulated by the Atrp and Chk1p checkpoint kinases in metazoans. In Saccharomyces cerevisiae, these checkpoints are regulated by the Atrp homologue Mec1p and the kinase Rad53p. A conserved role of these checkpoints is to block mitotic progression until DNA replication and repair are completed. In S. cerevisiae, these checkpoints include a transcriptional response regulated by the kinase Dun1p; however, dun1Delta cells are proficient for the S-phase-checkpoint-induced anaphase block. Yeast Chk1p kinase regulates the metaphase-to-anaphase transition in the DNA-damage checkpoint pathway via securin (Pds1p) phosphorylation. However, like Dun1p, yeast Chk1p is not required for the S-phase-checkpoint-induced anaphase block. Here we report that Chk1p has a role in the intra-S-phase checkpoint activated when yeast cells replicate their DNA in the presence of low concentrations of hydroxyurea (HU). Chk1p was modified and Pds1p was transiently phosphorylated in this response. Cells lacking Dun1p were dependent on Chk1p for survival in HU, and chk1Delta dun1Delta cells were defective in the recovery from rep...Continue Reading

References

May 23, 1998·Science·M N BoddyP Russell
Nov 21, 2001·Nature Cell Biology·A A AlcasabasS J Elledge
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Xiaolan Zhao, Rodney Rothstein
Nov 14, 2002·Annual Review of Genetics·Kara A NybergTed A Weinert
Jan 30, 2003·The EMBO Journal·George ZachosDavid A F Gillespie
May 29, 2003·Molecular Cell·José Antonio TerceroJohn F X Diffley
Aug 30, 2003·The Journal of Biological Chemistry·Ritu AgarwalOrna Cohen-Fix

❮ Previous
Next ❯

Citations

Nov 6, 2008·The Journal of Biological Chemistry·Yinhuai ChenYolanda Sanchez
Mar 31, 2006·Molecular Biology of the Cell·Hong Liu, Yanchang Wang
Oct 28, 2009·Molecular and Cellular Biology·Andrew C Kile, Deanna M Koepp
Mar 19, 2008·The Journal of Cell Biology·Julie M CaldwellYolanda Sanchez
Feb 18, 2005·The Journal of Cell Biology·Peter MeisterGiuseppe Baldacci
Jul 29, 2004·DNA Repair·Yinhuai Chen, Yolanda Sanchez
Jul 12, 2008·FEMS Microbiology Reviews·Yu FuWei Xiao
Mar 15, 2005·Yeast
Dec 13, 2006·Journal of Cell Science·Robert N WoolstencroftMike Tyers

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.