PMID: 9008707Jan 13, 1997Paper

A role for the actin cytoskeleton of Saccharomyces cerevisiae in bipolar bud-site selection

The Journal of Cell Biology
S YangD G Drubin

Abstract

Saccharomyces cerevisiae cells select bud sites according to one of two predetermined patterns. MATa and MAT alpha cells bud in an axial pattern, and MATa/alpha cells bud in a bipolar pattern. These budding patterns are thought to depend on the placement of spatial cues at specific sites in the cell cortex. Because cytoskeletal elements play a role in organizing the cytoplasm and establishing distinct plasma membrane domains, they are well suited for positioning bud-site selection cues. Indeed, the septin-containing neck filaments are crucial for establishing the axial budding pattern characteristic of MATa and MAT alpha cells. In this study, we determined the budding patterns of cells carrying mutations in the actin gene or in genes encoding actin-associated proteins: MATa/alpha cells were defective in the bipolar budding pattern, but MATa and MAT alpha cells still exhibit a normal axial budding pattern. We also observed that MATa/alpha actin cytoskeleton mutant daughter cells correctly position their first bud at the distal pole of the cell, but mother cells position their buds randomly. The actin cytoskeleton therefore functions in generation of the bipolar budding pattern and is required specifically for proper selection of...Continue Reading

References

Jan 1, 1976·Annual Review of Biochemistry·J A Snyder, J R McIntosh
Oct 1, 1992·Genetics·K F WertmanD Botstein
Aug 9, 1991·Cell·R Li, A W Murray
Jan 1, 1991·Methods in Enzymology·J R PringleB K Haarer
Jan 1, 1991·Methods in Enzymology·J R Pringle
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A Bender, J R Pringle
Oct 1, 1988·The Journal of Cell Biology·C W JacobsJ R Pringle
Dec 1, 1988·The Journal of Cell Biology·D G DrubinD Botstein
Jun 1, 1988·The Journal of Cell Biology·T C HuffakerD Botstein
Nov 1, 1987·The Journal of Cell Biology·A A Hyman, J G White
Mar 1, 1984·The Journal of Cell Biology·J V Kilmartin, A E Adams
May 1, 1995·The Journal of Cell Biology·J Chant, J R Pringle
May 1, 1995·The Journal of Cell Biology·J ChantJ R Pringle
Sep 1, 1994·Molecular Biology of the Cell·S J KronG R Fink
Apr 1, 1994·The Journal of Cell Biology·J MulhollandD Botstein
Jul 1, 1993·The Journal of Cell Biology·E G FlescherM Snyder
Jun 1, 1993·The Journal of Cell Biology·S N Hird, J G White
Feb 1, 1996·Current Opinion in Cell Biology·M S LongtineJ R Pringle
Oct 1, 1960·Journal of Bacteriology·D FREIFELDER

❮ Previous
Next ❯

Citations

Dec 20, 2002·Biology of the Cell·Rustem UzbekovClaude Prigent
Dec 4, 2001·Current Opinion in Microbiology·F YarmD Pellman
Apr 6, 2002·Current Opinion in Microbiology·Antonio Casamayor, Michael Snyder
Mar 6, 1999·Current Opinion in Microbiology·S J Palmieri, B K Haarer
Mar 6, 1999·Current Opinion in Microbiology·F Verde
Jan 19, 2000·Molecular Biology of the Cell·M C LorenzJ Heitman
May 22, 2001·Molecular Biology of the Cell·A S GladfelterD J Lew
Aug 22, 2001·Molecular Biology of the Cell·H A HarkinsJ R Pringle
Jul 11, 1998·Molecular Biology of the Cell·B M CaliG R Fink
Jan 6, 2006·Molecular Biology of the Cell·Helena FriesenBrenda Andrews
Jun 18, 2002·The EMBO Journal·Roland Wedlich-SöldnerGero Steinberg
Dec 3, 2004·Plant & Cell Physiology·Ja Choon KooMoo Je Cho
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Brian D SlaughterRong Li
Nov 29, 2002·Eukaryotic Cell·U OberholzerM Whiteway
Feb 7, 2001·Molecular and Cellular Biology·C M AslesonJ Berman
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Hay-Oak Park, Erfei Bi
Mar 10, 2006·Microbiology and Molecular Biology Reviews : MMBR·Gang RenAlan L Munn
Oct 6, 1998·Annual Review of Biochemistry·E CabibT Drgon
Dec 28, 1999·Annual Review of Cell and Developmental Biology·J Chant
Jan 19, 1999·Annual Review of Microbiology·K Madden, M Snyder
Jun 16, 2012·Genetics·Erfei Bi, Hay-Oak Park
Mar 23, 1999·The Journal of Cell Biology·R A Heil-ChapdelaineJ A Cooper
Mar 23, 1999·The Journal of Cell Biology·L LeeD Pellman
Feb 28, 1998·The Journal of Cell Biology·R K Miller, M D Rose
Feb 7, 2001·The Journal of Cell Biology·S Song, K S Lee
Jul 28, 2011·The EMBO Journal·Cornelia Kilchert, Anne Spang
Nov 28, 2000·Annual Review of Genetics·K D'HondtH Riezman
Aug 26, 1998·Current Biology : CB·K R Ayscough, D G Drubin

❮ Previous
Next ❯

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