Abstract
We have investigated the requirement of neutrophil emigration and the role for CD11b/CD18-mediated events in the experimental induction of acute lung injury. BALB/c mice received lipopolysaccharide (LPS) (3 mg/kg) intravenously (i.v.) 2 h prior to i.v. zymosan (10 mg/kg) and extravascular albumin accumulation was assessed after 30 min. Compared with saline-treated controls, zymosan alone caused a 6-fold increase in the accumulation of 125I-human serum albumin in whole lung tissue (P<0.05). Combined treatment with LPS and zymosan further increased extravascular albumin accumulation (P<0.05 compared with zymosan alone). The monoclonal antibody 5C6, directed against murine CD11b, was injected, 1 mg i.v. 15 min prior to LPS or 15 min before the zymosan, and compared with immunoglobulin G-injected controls. Albumin accumulation was significantly reduced by 5C6 when given prior to the LPS (P<0.01), but not when given before zymosan in the combined LPS and zymosan treatment. Interestingly, albumin accumulation induced by zymosan alone was not reduced by 5C6. The lungs of the mice treated with LPS and zymosan showed a marked, diffuse accumulation of inflammatory cells which, by light microscopy, appeared to be interstitial. Foci of neu...Continue Reading
Citations
Aug 7, 2012·Journal of Anesthesia·Yu-Li PangYu-Chuan Tsai
Jun 16, 2000·Journal of Immunological Methods·H M DavisD E Griswold
May 26, 1999·Infectious Disease Clinics of North America·C Parent, P Q Eichacker
May 30, 2001·Critical Care Medicine·N OhtaI Yoshiya
Jan 28, 1998·American Journal of Respiratory and Critical Care Medicine·Y T LuT W Evans
Feb 26, 1998·American Journal of Respiratory Cell and Molecular Biology·J M MiotlaP G Hellewell
Jun 17, 1998·The Journal of Clinical Investigation·M Y ZhouA B Malik
Jan 31, 2012·European Journal of Cell Biology·Lucy Kathleen ReissStefan Uhlig
Oct 2, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J S ErjefältC G Persson
Mar 23, 2001·Nature Immunology·T NagaseT Shimizu
Sep 15, 2001·Journal of Applied Microbiology·S FaddaF Toldrá
Mar 5, 2002·Clinical and Diagnostic Laboratory Immunology·Carmen FiuzaJose M Tellado
Dec 31, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Takahide NagaseTakao Shimizu
Jan 7, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kieran P O'DeaMasao Takata
Dec 7, 2010·Expert Review of Respiratory Medicine·Hong ChenXiangdong Wang
Jun 15, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kai HeckelAlwin E Goetz
Jan 9, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·James C Parker, Mary I Townsley
Dec 10, 1999·Journal of the American Society of Nephrology : JASN·A S De VrieseM W Steinhausen
May 5, 2001·The American Journal of Pathology·J A BurnsA C Issekutz