A role for the common GTP-binding protein in coupling of chromosome replication to cell growth and cell division

Biochemical and Biophysical Research Communications
Aleksandra Sikora-BorgulaAgata Czyz

Abstract

Homologues of CgtA, the common GTP-binding protein of Vibrio harveyi, are present in diverse organisms ranging from bacteria to humans. In bacteria, proteins homologous to CgtA form a subfamily of small GTP-binding proteins, called Obg/Gtp1. Similarity between bacterial members of this subfamily and their eukaryotic homologues is as high as about 50%. Nevertheless, specific functions of these proteins remain largely unknown. Genes coding for CgtA-like proteins are essential in almost all species of bacteria. The only known exception is V. harveyi, whose cells survive disruption of the cgtA gene. Therefore, the V. harveyi cgtA insertional mutant is a very useful tool for studies on functions of CgtA. Here we demonstrate that under normal growth conditions, cells of the cgtA mutant are slightly larger than wild-type cells, whereas indirect inhibition of DNA replication initiation by addition of rifampicin results in significantly higher differences in average cell size between these two strains as measured by flow cytometry. These differences decreased when cell division was inhibited by cephalexin. DNA synthesis per cell mass was found to be increased in the cgtA mutant relative to wild-type V. harveyi strain, whereas the mutant...Continue Reading

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Citations

Jan 2, 2004·Journal of Bacteriology·Bin LinJanine R Maddock
Nov 11, 2014·Metabolomics : Official Journal of the Metabolomic Society·Xuan WangJian Zhi Hu
Dec 14, 2005·Biochemical and Biophysical Research Communications·A E SikoraJ R Maddock
Apr 12, 2005·Plant Molecular Biology·Emidio AlbertiniMario Falcinelli
Jul 26, 2008·Applied Microbiology and Biotechnology·Juntaek LimSeokhwan Hwang
Jun 27, 2009·Molecular Microbiology·Nicole S PerskySusan T Lovett
Feb 2, 2021·Frontiers in Cellular and Infection Microbiology·Wolfgang EisenreichWerner Goebel
Mar 22, 2019·Archives of Biochemistry and Biophysics·Ananya ChatterjeePartha P Datta
Feb 22, 2005·Molecular Cell·James J FotiSusan T Lovett

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