A role for the PERIOD:PERIOD homodimer in the Drosophila circadian clock

PLoS Biology
Johannes LandskronRalf Stanewsky


Circadian clocks in eukaryotes rely on transcriptional feedback loops, in which clock genes repress their own transcription resulting in molecular oscillations with a period of approximately 24 h. In Drosophila, the clock proteins Period (PER) and Timeless (TIM) operate in such a feedback loop, whereby they first accumulate in the cytoplasm of clock cells as a heterodimer. Nuclear translocation of the complex or the individual PER and TIM proteins is followed by repression of per and tim transcription, whereby PER seems to act as the prime repressor. We found that in addition to PER:TIM complexes, functional PER:PER homodimers exist in flies. Specific disruption of PER homodimers results in drastically impaired behavioral and molecular rhythmicity, pointing the biological importance of this clock protein complex. Analysis of PER subcellular distribution and repressor competence in the PER dimer mutant revealed defects in PER nuclear translocation and a disruption of rhythmic period transcription. The striking similarity of these phenotypes with that of reduced CKII activity suggests that the formation or function of the PER dimer is closely linked to this kinase. Our results confirm a previous structural model for PER and provi...Continue Reading


Feb 1, 1992·Genome Génome / Conseil National De Recherches Canada·C Magoulas, D A Hickey
Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·C PlassH Winking
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·R J Konopka, S Benzer
Oct 22, 1982·Science·G M Rubin, A C Spradling
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·I EderyM Rosbash
Jun 1, 1997·Journal of Biological Rhythms·J D PlautzS A Kay
Jul 1, 1997·Molecular and Cellular Biology·H HaoP E Hardin
Apr 8, 1998·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·C Helfrich-Förster
Oct 23, 1998·Journal of Biological Rhythms·J E RutilaM Rosbash
Oct 10, 2001·The Journal of Biological Chemistry·E L VielhaberD M Virshup
Mar 16, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yasufumi ShigeyoshiHitoshi Okamura
Nov 26, 2002·Nature·Jui-Ming LinRavi Allada
Dec 18, 2002·Journal of Neurobiology·Ralf Stanewsky
Feb 4, 2003·Nature Neuroscience·Bikem AktenF Rob Jackson
May 3, 2003·Current Biology : CB·Dennis C Chang, Steven M Reppert
Oct 17, 2003·Current Biology : CB·Shobi VeleriRalf Stanewsky
Mar 19, 2004·Current Biology : CB·Orie T ShaferJeffery C Hall

❮ Previous
Next ❯


Jun 7, 2014·Annual Review of Biochemistry·Brian R Crane, Michael W Young
Sep 17, 2009·Journal of Biological Rhythms·Valerie L Kilman, Ravi Allada
Aug 4, 2010·Journal of Biological Rhythms·Ralph Jürgen StelzerLars Chittka
Feb 6, 2013·Journal of Biological Rhythms·Pamela MenegazziRodolfo Costa
Aug 12, 2009·Journal of Neurochemistry·Changtaek ChoiJoonho Choe
Jun 6, 2013·Journal of Biological Rhythms·Yue Li, Michael Rosbash
Jun 11, 2011·The Journal of Biological Chemistry·Sheyum SyedMichael W Young
Aug 9, 2011·Essays in Biochemistry·Nicholas R J Glossop

❮ Previous
Next ❯

Methods Mentioned

nuclear translocation
gel filtration
in vivo
protein blots

Software Mentioned

BRASS ( biological rhythms analysis

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.