A role for the Rcs phosphorelay in regulating expression of plant cell wall degrading enzymes in Pectobacterium carotovorum subsp. carotovorum

Microbiology
Liis AndresenAndres Mäe

Abstract

The Rcs phosphorelay is a signal transduction system that influences the virulence phenotype of several pathogenic bacteria. In the plant pathogen Pectobacterium carotovorum subsp. carotovorum (Pcc) the response regulator of the Rcs phosphorelay, RcsB, represses expression of plant cell wall degrading enzymes (PCWDE) and motility. The focus of this study was to identify genes directly regulated by the binding of RcsB that also regulate expression of PCWDE genes in Pcc. RcsB-binding sites within the regulatory regions of the flhDC operon and the rprA and rsmB genes were identified using DNase I protection assays, while in vivo studies using flhDC : : gusA, rsmB : : gusA and rprA : : gusA gene fusions revealed gene regulation. These experiments demonstrated that the operon flhDC, a flagellar master regulator, was repressed by RcsB, and transcription of rprA was activated by RcsB. Regulation of the rsmB promoter by RcsB is more complicated. Our results show that RcsB represses rsmB expression mainly through modulating flhDC transcription. Neverthless, direct binding of RcsB on the rsmB promoter region is possible in certain conditions. Using an rprA-negative mutant, it was further demonstrated that RprA RNA is not essential for re...Continue Reading

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Citations

Aug 21, 2010·Future Microbiology·David J Clarke
Jun 19, 2012·Annual Review of Phytopathology·Amy CharkowskiIris Yedidia
Sep 3, 2014·Molecular Plant-microbe Interactions : MPMI·Xiaogang WuChing-Hong Yang
Dec 27, 2011·Current Opinion in Microbiology·Yvonne Göpel, Boris Görke
Sep 10, 2016·Microbiology·Stéphanie BorlandFlorence Wisniewski-Dyé
May 14, 2019·BioMed Research International·Qian JiangKang Yao
Nov 8, 2020·Applied and Environmental Microbiology·Xuewei PanZhiming Rao

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