A role for the Salmonella Type III Secretion System 1 in bacterial adaptation to the cytosol of epithelial cells

Molecular Microbiology
Audrey ChongOlivia Steele-Mortimer

Abstract

Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that invades the intestinal epithelium. Following invasion of epithelial cells, Salmonella survives and replicates within two distinct intracellular niches. While all of the bacteria are initially taken up into a membrane bound vacuole, the Salmonella-containing vacuole or SCV, a significant proportion of them promptly escape into the cytosol. Cytosolic Salmonella replicates more rapidly compared to the vacuolar population, although the reasons for this are not well understood. SipA, a multi-function effector protein, has been shown to affect intracellular replication and is secreted by cytosolic Salmonella via the invasion-associated Type III Secretion System 1 (T3SS1). Here, we have used a multipronged microscopy approach to show that SipA does not affect bacterial replication rates per se, but rather mediates intra-cytosolic survival and/or initiation of replication following bacterial egress from the SCV. Altogether, our findings reveal an important role for SipA in the early survival of cytosolic Salmonella.

References

Nov 5, 1997·Veterinary Pathology·A J FrostT S Wallis
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·D ZhouJ E Galán
Mar 3, 2004·Molecular Cell·Emma J McGhieVassilis Koronakis
Feb 24, 2006·The Journal of Biological Chemistry·Cheryl L BirminghamJohn H Brumell
Mar 6, 2007·PLoS Pathogens·Andrea L RadtkeMary X D O'Riordan
Apr 5, 2008·PLoS Pathogens·Robert J CainVassilis Koronakis
Dec 31, 2008·Microbes and Infection·Josely F FigueiredoL Garry Adams
Apr 17, 2009·Nature Reviews. Microbiology·Katrina RayChristoph M Tang
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kailiang JiaBeth Levine
Dec 3, 2009·Cellular Microbiology·Irit PazPhilippe Sansonetti
Sep 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Leigh A KnodlerOlivia Steele-Mortimer
Jan 24, 2012·Methods in Enzymology·Samantha StehbensTorsten Wittmann
Jun 22, 2012·PLoS Pathogens·Francisco S MesquitaDavid W Holden
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Jun 19, 2013·Cell Host & Microbe·Jamaal L BenjaminLora V Hooper
Jan 9, 2014·PloS One·Leigh A KnodlerOlivia Steele-Mortimer
Mar 10, 2015·Nature Reviews. Microbiology·Doris L LaRockSamuel I Miller
Nov 22, 2016·Trends in Molecular Medicine·Jacqueline M Kimmey, Christina L Stallings
Apr 21, 2017·PLoS Pathogens·Ciaran E FinnOlivia Steele-Mortimer
Dec 5, 2017·Frontiers in Cellular and Infection Microbiology·Kendal G CooperOlivia Steele-Mortimer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.