PMID: 9557717Apr 29, 1998Paper

A role for the Sendai virus P protein trimer in RNA synthesis

Journal of Virology
J Curran

Abstract

The SeV P protein is found as a homotrimer (P3) when it is expressed in mammalian cells, and trimerization is mediated by a predicted coiled-coil motif which maps within amino acids (aa) 344 to 411 (the BoxA region). The bacterially expressed protein also appears to be trimeric, apparently precluding a role for phosphorylation in the association of the P monomers. I have examined the role of P trimerization both in the protein's interaction with the nucleocapsid (N:RNA) template and in the protein's function on the template during RNA synthesis. As with the results of earlier experiments (32), I found that both the BoxA and BoxC (aa 479 to 568) regions were required for stable binding of P to the N:RNA. Binding was also observed with P proteins containing less than three BoxC regions, suggesting that trimerization may be required to permit contacts between multiple BoxC regions and the N:RNA. However, these heterologous trimers failed to function in viral RNA synthesis, indicating that the third C-terminal leg of the trimer plays an essential role in P function on the template. We speculate that this function may involve the movement of P (and possibly the polymerase complex) on the template and the maintenance of processivity.

References

Jan 1, 1976·Virology·R A LambP W Choppin
May 1, 1989·Journal of Virology·E H EgelmanG Murti
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
Nov 1, 1974·Journal of Virology·W E MountcastleP W Choppin

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Citations

Aug 24, 2013·Viruses·Tomoyuki Honda, Keizo Tomonaga
Sep 5, 2013·The Journal of Biological Chemistry·Stefanie A KrummRichard K Plemper
Aug 28, 2015·Journal of Virology·Adrian PickarBiao He
Jul 29, 2004·Journal of Virology·Richard L KingstonLeslie S Gay
Nov 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Pau BernadóMartin Blackledge
Apr 15, 2003·Clinical Microbiology Reviews·Kelly J Henrickson
Oct 22, 2019·Journal of Molecular Biology·Nathan E JespersenElisar Barbar
Jan 30, 2004·Virus Research·Achut G MalurAmiya K Banerjee

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