A role for Vibrio vulnificus PecS during hypoxia.

Scientific Reports
Nabanita BhattacharyyaAnne Grove

Abstract

The genus Vibrio includes serious human pathogens, and mollusks are a significant reservoir for species such as V. vulnificus. Vibrio species encode PecS, a member of the multiple antibiotic resistance regulator (MarR) family of transcription factors; pecS is divergently oriented to pecM, which encodes an efflux pump. We report here that Vibrio species feature frequent duplications of pecS-pecM genes, suggesting evolutionary pressures to respond to distinct environmental situations. The single V. vulnificus PecS binds two sites within the pecS-pecM intergenic region with Kd = 0.3 ± 0.1 nM, a binding that is attenuated by the ligands xanthine and urate, except when promoter DNA is saturated with PecS. A unique target is found in the intergenic region between genes encoding the nitric oxide sensing transcription factor, NsrR, and nod; the nod-encoded nitric oxide dioxygenase is important for preventing nitric oxide stress. Reporter gene assays show that PecS-mediated repression of gene expression can be relieved in presence of ligand. Since xanthine and urate are produced as part of the oxidative burst during host defenses and under molluscan hypoxia, we propose that these intermediates in the host purine degradation pathway func...Continue Reading

References

Jul 27, 2005·Biochemistry·Michael A MenzeManfred K Grieshaber
May 5, 2010·Nitric Oxide : Biology and Chemistry·Andrea PautzHartmut Kleinert
Aug 4, 2010·Journal of Molecular Biology·Inoka C Perera, Anne Grove
Oct 22, 2010·BMC Genomics·Marit S BratlieFinn Drabløs
Mar 25, 2011·Protein Science : a Publication of the Protein Society·Inoka C Perera, Anne Grove
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Mar 5, 2013·Biochimica Et Biophysica Acta·Jürgen Markl
Jul 9, 2014·Journal of Bacteriology·Robert S DwyerThomas J Silhavy
Dec 17, 2014·The Biochemical Journal·Hao HuangAnne Grove
Feb 18, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Brett A Froelich, Rachel T Noble
Jun 21, 2017·Marine Drugs·Carla ZannellaMassimiliano Galdiero
Jul 4, 2017·Critical Reviews in Biochemistry and Molecular Biology·Dinesh K Deochand, Anne Grove
Oct 14, 2017·Environmental Microbiology·Craig Baker-Austin, James D Oliver
Nov 1, 2017·ACS Chemical Biology·Ashish GuptaAnne Grove
Jun 12, 2018·Annual Review of Marine Science·Katja Fennel, Jeremy M Testa

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Methods Mentioned

BETA
size exclusion chromatography
electrophoretic mobility assay
footprinting
PCR
crosslinking assay
electrophoresis

Software Mentioned

Clustal Omega
SwissModel
RegPrecise
ImageQuant
Patloc
Sigma Plot
MUSCLE
iTOL
BLASTp
KaleidaGraph

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