A root acyl carrier protein-II from spinach is also expressed in leaves and seeds

Plant Molecular Biology
K M Schmid, J B Ohlrogge

Abstract

During the synthesis of fatty acids and their utilization in plastids, fatty acyl moieties are linked to acyl carrier protein (ACP). In contrast to previously cloned organ-specific ACP isoforms, we have now isolated a cDNA clone for a potentially constitutive ACP isoform from a spinach root library. Identity between the amino acid sequence encoded by this cDNA and N-terminal sequence data for ACP-II protein from spinach leaf indicates that the root cDNA encodes ACP-II. The deduced amino acid sequence for ACP-II shows 62% identity with spinach leaf ACP-I. Southern analysis suggests that multiple ACP genes or pseudogenes occur in the spinach genome. High-stringency northern blot analysis and RNase protection studies confirm that, within the region encoding the mature ACP-II, the cloned ACP sequence is expressed in leaves and seeds as well as in roots. Quantitative RNase protection data indicate that the ratio of ACP-I and ACP-II mRNA sequences in leaf is similar to the ratio of the two proteins.

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Citations

Jun 12, 1999·The Plant Journal : for Cell and Molecular Biology·M C SuhJ B Ohlrogge
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S L Wang, X Q Liu
Jun 25, 1991·Nucleic Acids Research
Feb 10, 1999·Plant Physiology·D HondredR D Vierstra
Jan 1, 1992·Plant Physiology·A Hlousek-RadojcićJ B Ohlrogge
Oct 1, 1991·Molecular & General Genetics : MGG·L Hansen, P von Wettstein-Knowles
Apr 5, 1991·Science·C Somerville, J Browse

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