A Salmonella-based, propionate-inducible, expression system for Salmonella enterica

Gene
Sung Kuk Lee, Jay D Keasling

Abstract

The expression and regulatory properties of a propionate-regulated overexpression system (Salmonella enterica prpBCDE promoter (P(prpB)) and positive regulator (prpR)) were evaluated in wild-type S. enterica serovar Typhimurium TR6583 and prpB(-) or prpD(-) versions of this strain and compared with the arabinose-regulated T7 expression system. The wild-type strain showed low expression in the absence of propionate and high expression in the presence of propionate under all growth conditions. In 96-well plates and culture tubes, the wild-type strain exhibited a long delay before full induction; the time delay was significantly shorter in shake flasks. The prpD(-) strain exhibited low expression in the presence of glucose, highly regulatable expression over a wide range of propionate concentrations, and, in contrast to the wild-type strain, fast induction to full expression under all growth conditions. In contrast, the prpB(-) strain showed very high background expression in both culture tubes and shake flasks.

References

Jan 1, 1982·Molecular & General Genetics : MGG·J H LeeG Wilcox
Apr 1, 1996·FEMS Microbiology Letters·T A HammelmanJ C Escalante-Semerena
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·D A Siegele, J C Hu
Dec 28, 1999·Analytical Biochemistry·D R Wycuff, K S Matthews
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
May 29, 2001·The Journal of Biological Chemistry·A R HorswillJ C Escalante-Semerena
Aug 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·L Reitzer, B L Schneider
Oct 11, 2002·Journal of Bacteriology·Jeffrey McKinneySidney Altman
Nov 5, 2005·Applied and Environmental Microbiology·Sung Kuk Lee, Jay D Keasling
Dec 8, 2005·Biotechnology and Bioengineering·Sung Kuk Lee, Jay D Keasling

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Citations

Nov 13, 2018·Frontiers in Microbiology·Min Ju Lee, Pil Kim
Dec 22, 2016·Applied Microbiology and Biotechnology·Lukas MarschallChristoph Herwig
Mar 18, 2021·Advanced Biology·Yang ZhangShuobo Shi
Jan 22, 2008·ACS Chemical Biology·Jay D Keasling

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