PMID: 7799834Jan 1, 1994

A sandwich cup method for the penetration assay of antimicrobial agents through Pseudomonas exopolysaccharides

Microbiology and Immunology
H KumonH Ohmori

Abstract

We developed new sandwich cup method to assay the penetration of various antimicrobial agents through Pseudomonas exopolysaccharides. Using alginate extracted from mucoid-type Pseudomonas aeruginosa and gellan gum from Pseudomonas elodea, the role of exopolysaccharides as a barrier against drug penetration was examined. The penetration of positively charged hydrophilic drugs such as aminoglycosides and polypeptides was markedly inhibited by the gels tested, but that of beta-lactams, quinolones, and macrolides was not inhibited. The penetration of gentamicin was strongly influenced by the gel concentration, the solution to be used, and the presence of Ca2+. These results suggest that the microenvironment at the infection site could greatly influence drug penetration through biofilms in vivo.

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Related Concepts

Gelrite
EPS I, Pseudomonas
Alginates
Bacteriocidal Agents
Gel
Hexuronic Acids
Fungus Drug Sensitivity Tests
Polysaccharides, Bacterial
Pseudomonas aeruginosa
Glucuronic Acid, 6-(14)C-labeled, (D)-isomer

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