PMID: 8591834Nov 30, 1995Paper

A sandwich enzyme-linked immunosorbent assay for human seminal gamma-glutamyl transpeptidase

Forensic Science International : Synergy
S AbeK Hiraiwa

Abstract

A sandwich enzyme-linked immunosorbent assay (ELISA) was developed for detecting human seminal gamma-glutamyl transpeptidase (gamma-GTP) using a combination of anti-seminal gamma-GTP monoclonal antibodies. These monoclonal antibodies did not react with human ovary or uterus in immunohistochemical study. Optimal assay condition, resulting in a sensitive assay with a low background, is presented. The detection limit of this assay was estimated to be 1 ng/ml of seminal gamma-GTP corresponding to about 100,000 times dilution of seminal sample. This ELISA was specific for seminal gamma-GTP, without cross-reactivity to renal or hepatic gamma-GTP, normal blood serum, non-coital vaginal fluid or saliva. The recovery of seminal gamma-GTP added to various biological fluids were also examined.

References

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Citations

Jul 15, 1999·Clinica Chimica Acta; International Journal of Clinical Chemistry·S AbeK Hiraiwa
Mar 11, 1998·Forensic Science International : Synergy·S AbeK Hiraiwa
Jun 15, 1997·Analytical Chemistry·T A Brettell, R Saferstein

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