PMID: 649961Jan 1, 1978

A sandwich method of enzyme-immunoassay. II. Quantification of rheumatoid factor

Journal of Immunological Methods
R MaioliniR Massaeyeff


A sandwich enzyme-immunoassay (EIA) has been applied to the determination of the rheumatoid factor (RF). This non-competeitive assay comprises 3 steps: 1) the RF to be assayed is extracted for the biological medium by an immunosorbent of aggregated IgG linked to cellulose; 2) the solid phase is then incubated with the enzyme-labeled aggregated IgG; 3) the enzymatic activity of the immunosorbent is then measured with a suitable chromogenic reagent. This activity is a direct function of the amount of RF to be assayed. This assay gave reproducible results in the range 0.5-50.0 IU/ml. A good agreement was obtained between the EIA and the Waaler-Rose test but no correlation was obtained with the latex slide-test. This assay permits a quantitation of RF with a good reproducibility (coefficient of variation in the range of 10% for moderately elevated values) and thus allows a closer follow-up of patients. The results do not depend on the interpretation of the technician performing the test, which can be easily automated. Finally, it may detect some RF devoid of agglutinating activity.


Sep 1, 1975·Journal of Immunological Methods·R Maiolini, R Masseyeff
Oct 1, 1969·Arthritis and Rheumatism·P Franchimont, S Suteanu
Feb 1, 1961·Proceedings of the Society for Experimental Biology and Medicine·H J MULLER-EBERHARD, H G KUNKEL
May 1, 1948·Proceedings of the Society for Experimental Biology and Medicine·H M ROSE, C RAGAN


Jan 1, 1986·Rheumatology International·T JonssonH Valdimarsson
Apr 16, 1982·Molecular and Cellular Biochemistry·T T Ngo, H M Lenhoff
Jan 1, 1979·Journal of Immunological Methods·B FerruaR Masseyeff
Jan 1, 1979·Journal of Immunological Methods·M GripenbergE Linder
Jan 1, 1980·Journal of Immunological Methods·J KarshA D Steinberg
Dec 30, 1983·Journal of Immunological Methods·R A Togun, P Brenchley
Sep 27, 1986·Journal of Immunological Methods·M Gripenberg, P Kurki
Feb 24, 1988·Journal of Immunological Methods·P L GigaseW J Stevens
Dec 1, 1993·Veterinary Immunology and Immunopathology·L ChabanneJ C Monier
Feb 25, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·H YamanishiT Yanagihara
Jan 1, 1985·Journal of Immunoassay·B Ferrua, R Masseyeff
Jan 1, 1987·Annals of the Rheumatic Diseases·M Gioud-PaquetJ C Monier
May 1, 1988·Immunological Investigations·M ZreinM H Van Regenmortel
Jan 1, 1979·Scandinavian Journal of Rheumatology·M VejtorpP Halberg
Jan 1, 1987·The International Journal of Social Psychiatry·S C BhatiaA Sharma
Dec 1, 1981·Genetical Research·A Putrament, A Ejchart
Jun 1, 1980·Acta Pathologica Et Microbiologica Scandinavica. Section C, Immunology·B Ziola, H Tuokko
Jun 1, 1979·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·M VejtorpJ Leerhoy
Oct 1, 1985·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology·M KempS E Svehag
Sep 1, 1979·Annals of Clinical Biochemistry·M J O'SullivanV Marks

Related Concepts

Antibody Specificity
Enzyme-Linked Immunosorbent Assay
Chinchilla Rabbits
Rheumatoid Factor

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.